HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF D-AMINO-ACID OXIDASE ACTIVITY

被引：6

作者：

BIONDI, PA

GUIDOTTI, L

NEGRI, A

SECCHI, C

机构：

[1] Institute of Veterinary Physiology and Biochemistry, 20133 Milan

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 566卷 / 02期

关键词：

D O I：

10.1016/0378-4347(91)80254-A

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new procedure for the assay of D-amino acid oxidase activity has been developed. Alpha-Ketoisovaleric acid, derived from D-valine, was estimated by high-performance liquid chromatography after reaction with omicron-phenylenediamine to give the corresponding quinoxalinol derivative. Alpha-Ketovaleric acid was used as an internal standard to ensure the reproducibility of the method. As an example of application, kidney cortex homogenates were analyzed for their D-amino acid oxidase activity. The advantages of the presented procedure for the determination of the enzymatic activity in biological samples compared with previously reported procedures are discussed.

引用

页码：377 / 382

页数：6

共 12 条

[1] D-AMINO ACID OXIDASE .2. SPECIFICITY COMPETITIVE INHIBITION AND REACTION SEQUENCE [J].