SIGMA-RECEPTORS AND OPIOID RECEPTORS IN HUMAN BRAIN-TUMORS

被引：69

作者：

THOMAS, GE ^{[1
]}

SZUCS, M ^{[1
]}

MAMONE, JY ^{[1
]}

BEM, WT ^{[1
]}

RUSH, MD ^{[1
]}

JOHNSON, FE ^{[1
]}

COSCIA, CJ ^{[1
]}

机构：

[1] ST LOUIS UNIV,SCH MED,DEPT SURG,ST LOUIS,MO 63104

来源：

LIFE SCIENCES | 1990年 / 46卷 / 18期

关键词：

D O I：

10.1016/0024-3205(90)90360-4

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Human brain tumors (obtained as surgical specimens) and nude mouse-borne human neuroblastomas and gliomas were analyzed for sigma and opioid receptor content. Sigma binding was assessed using [3H] 1, 3-di-o-tolylguanidine (DTG), whereas opioid receptor subtypes were measured with tritiated forms of the following: μ, [D-ala2, mePhe4, gly-ol5] enkephalin (DAMGE); κ, ethylketocyclazocine (EKC) or U69, 593; δ, [D-pen2, D-pen5] enkephalin (DPDPE) or [D-ala2, D-leu5] enkephalin (DADLE) with μ suppressor present. Binding parameters were estimated by homologous displacement assays followed by analysis using the LIGAND program. Sigma binding was detected in 15 of 16 tumors examined with very high levels (pmol/mg protein) found in a brain metastasis from an adenocarcinoma of lung and a human neuroblastoma (SK-N-MC) passaged in nude mice. κ opioid receptor binding was detected in 4 of 4 glioblastoma multiforme specimens and 2 of 2 human astrocytoma cell lines tested but not in the other brain tumors analyzed. © 1990.

引用

页码：1279 / 1286

页数：8

共 31 条

[1] SIGMA-RECEPTORS NEGATIVELY MODULATE AGONIST-STIMULATED PHOSPHOINOSITIDE METABOLISM IN RAT-BRAIN [J].