INFRASPECIFIC AND INTRASPECIFIC CLASSIFICATION OF PSEUDOMONAS-SOLANACEARUM STRAINS, USING WHOLE CELL FATTY-ACID ANALYSIS

被引:41
作者
JANSE, JD
机构
[1] Department of Bacteriology, Plant Protection Service, Wageningen
关键词
GLC; MULTIVARIATE ANALYSIS; AVIRULENT STRAINS; PSEUDOMONAS-CARYOPHYLLI; PSEUDOMONAS-CEPACIA; PSEUDOMONAS-GLADIOLI PV ALLIICOLA; PSEUDOMONAS-GLADIOLI PV GLADIOLI; PSEUDOMONAS-PICKETTII;
D O I
10.1016/S0723-2020(11)80307-3
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Whole cell fatty acid patterns of 69 strains of the plant pathogenic bacterium Pseudomonas solanacearum were determined by capillary gas liquid chromatography (GLC). Strains originated from 17 different hosts from many parts of the world covering four biovars and three (pathogenic) races as well as Zingiber strains. Identity of strains was confirmed by conventional biochemical and pathogenicity tests. Multivariate analysis of fatty acid data produced four infraspecific clusters. Three of these clusters corresponded to races 1, 2 and 3, the fourth consisted of five deviating race 1 biovar 1 strains from the U.S.A. Biovar 4 strains of Zingiber were found in the race 1 cluster. Only one biovar 3 strain from this host behaved differently. In contrast to conventional tests it was possible to discriminate between avirulent strains of different races. From unknown strains the race can be determined, using a reference library. The use of the term 'pathovar' instead of 'race' is discussed. Intraspecific relationships between P. solanacearum and four related species (P. caryophylli, P. cepacia, P. gladioli, P. pickettii) of rRNA group II were studied also, using fatty acid analysis. The taxonomic pattern found in DNA-DNA homology studies of these species was confirmed.
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页码:335 / 345
页数:11
相关论文
共 38 条
[1]   TAXONOMY OF AEROBIC PSEUDOMONADS - PSEUDOMONAS-CEPACIA,PSEUDOMONAS-MARGINATA,PSEUDOMONAS-ALLIICOLA AND PSEUDOMONAS-CARYOPHYLLI [J].
BALLARD, RW ;
PALLERONI, NJ ;
DOUDOROFF, M ;
STANIER, RY ;
MANDEL, M .
JOURNAL OF GENERAL MICROBIOLOGY, 1970, 60 :199-+
[2]  
BRADBURY JF, 1986, GUIDE PLANT PATHOGEN
[3]  
BUDDENHAGEN I, 1962, PHYTOPATHOLOGY, V52, P726
[4]  
Buddenhagen I. W., 1986, BACTERIAL WILT DIS A, P126
[5]  
BUDDENHAGEN IVAN, 1964, ANN REV PHYTOPATHOL, V2, P203, DOI 10.1146/annurev.py.02.090164.001223
[6]  
COOK D, 1989, Molecular Plant-Microbe Interactions, V2, P113, DOI 10.1094/MPMI-2-113
[7]   DIFFERENTIATION OF ERWINIA-CAROTOVORA SSP CAROTOVORA AND ERWINIA-CAROTOVORA SSP ATROSEPTICA ON THE BASIS OF CELLULAR FATTY-ACID COMPOSITION [J].
DEBOER, SH ;
SASSER, M .
CANADIAN JOURNAL OF MICROBIOLOGY, 1986, 32 (10) :796-800
[8]  
Dye D. W., 1980, Review of Plant Pathology, V59, P153
[9]   NUMERICAL-ANALYSIS OF PHENOTYPIC FEATURES OF PSEUDOMONAS-SOLANACEARUM STRAINS ISOLATED FROM TOBACCO AND OTHER HOSTS IN SOUTH-AFRICA [J].
ENGELBRECHT, MC ;
HATTINGH, MJ .
PLANT DISEASE, 1989, 73 (11) :893-898
[10]   STRAINS OF PSEUDOMONAS-SOLANACEARUM FROM CENTRAL-AMERICA AND SOUTH-AMERICA - A COMPARATIVE STUDY [J].
FRENCH, ER ;
SEQUEIRA, L .
PHYTOPATHOLOGY, 1970, 60 (03) :506-&