THE DNA ADENINE METHYLTRANSFERASE-ENCODING GENE (DAM) OF VIBRIO-CHOLERAE

被引：16

作者：

BANDYOPADHYAY, R ^{[1
]}

DAS, J ^{[1
]}

机构：

[1] INDIAN INST CHEM BIOL,DIV BIOPHYS,CALCUTTA 700032,W BENGAL,INDIA

来源：

GENE | 1994年 / 140卷 / 01期

关键词：

SPONTANEOUS MUTATION; DNA MISMATCH REPAIR; STRAND DISCRIMINATION; CLONING; EXPRESSION; BASE ANALOGS; NUCLEOTIDE SEQUENCE;

D O I：

10.1016/0378-1119(94)90732-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The DNA adenine methyltransferase (MTase)-encoding gene (dam) of Vibrio cholerae, an organism belonging to the family Vibrionaceae, has been cloned and the complete nucleotide (nt) sequence determined. V. cholerae dam encodes a 21.5-kDa protein and is directly involved in methyl-directed DNA mismatch repair. It can substitute for the Escherichia coli enzyme and can suppress the phenotypic traits associated with E. coli dam mutants. Overproduction of V. cholerae Dam MTase does not result in hypermutability in either V. cholerae or E. coli cells. Overproduction of V. cholerae Dam in a pUC plasmid, however, fails to suppress the 2-aminopurine (2-AP)-sensitive phenotype of E. coli dam mutants. Homology between the nt and deduced amino acid (aa) sequences of the E. coli and V. cholerae dam genes is only 30-35%.

引用

页码：67 / 71

页数：5

共 19 条

[1] CHARACTERIZATION OF DNA ADENINE METHYLATION MUTANTS OF ESCHERICHIA-COLI-K12 [J].

BALE, A ;

DALARCAO, M ;

MARINUS, MG .

MUTATION RESEARCH, 1979, 59 (02) :157-165

[2] A MUTATION IN THE DAM GENE OF VIBRIO-CHOLERAE - 2-AMINOPURINE SENSITIVITY WITH INTACT GATC METHYLASE ACTIVITY [J].

BANDYOPADHYAY, R ;

SENGUPTA, A ;

DAS, J .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 165 (02) :561-567

[3]

CLAVERYS JP, 1986, MICROBIOL REV, V50, P133, DOI 10.1128/MMBR.50.2.133-165.1986

[4] INDUCTION OF DAMAGE INDUCIBLE (SOS) REPAIR IN DAM MUTANTS OF ESCHERICHIA-COLI EXPOSED TO 2-AMINOPURINE [J].

CRAIG, RJ ;

ARRAJ, JA ;

MARINUS, MG .

MOLECULAR & GENERAL GENETICS, 1984, 194 (03) :539-540

[5] INDUCED MUTAGENESIS IN DAM[-]MUTANTS OF ESCHERICHIA-COLI - ROLE FOR 6-METHYLADENINE RESIDUES IN MUTATION AVOIDANCE [J].

GLICKMAN, B ;

VANDENELSEN, P ;

RADMAN, M .

MOLECULAR AND GENERAL GENETICS, 1978, 163 (03) :307-312

[6] SEQUENCE SPECIFICITY OF THE P1 MODIFICATION METHYLASE (M.ECO P1) AND THE DNA METHYLASE (M.ECO DAM) CONTROLLED BY THE ESCHERICHIA-COLI-DAM GENE [J].

HATTMAN, S ;

BROOKS, JE ;

MASUREKAR, M .

JOURNAL OF MOLECULAR BIOLOGY, 1978, 126 (03) :367-380

[7] ESCHERICHIA-COLI K-12 CLONES THAT OVERPRODUCE DAM METHYLASE ARE HYPERMUTABLE [J].

HERMAN, GE ;

MODRICH, P .

JOURNAL OF BACTERIOLOGY, 1981, 145 (01) :644-646

[8] COMPLEMENTARY SPECIFICITY OF RESTRICTION ENDONUCLEASES OF DIPLOCOCCUS-PNEUMONIAE WITH RESPECT TO DNA METHYLATION [J].

LACKS, S ;

GREENBERG, B .

JOURNAL OF MOLECULAR BIOLOGY, 1977, 114 (01) :153-168

[9] CORRELATION OF DNA ADENINE METHYLASE ACTIVITY WITH SPONTANEOUS MUTABILITY IN ESCHERICHIA-COLI-K-12 [J].

MARINUS, MG ;

POTEETE, A ;

ARRAJ, JA .

GENE, 1984, 28 (01) :123-125

[10] HYPER-RECOMBINATION IN DAM MUTANTS OF ESCHERICHIA-COLI K-12 [J].

MARINUS, MG ;

KONRAD, EB .

MOLECULAR & GENERAL GENETICS, 1976, 149 (03) :273-277

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