FULLY MECHANIZED LATEX IMMUNOASSAY FOR SERUM LIPOPROTEIN(A)

被引：17

作者：

ABE, A

YOSHIMURA, Y

SEKINE, T

MAEDA, S

YAMASHITA, S

NOMA, A

机构：

[1] GIFU UNIV,SCH MED,DEPT LAB MED,GIFU 500,JAPAN

[2] TOKUYAMA SODA CO LTD,TSUKUBA RES LABS,TSUKUBA,IBARAKI,JAPAN

来源：

CLINICA CHIMICA ACTA | 1994年 / 225卷 / 02期

关键词：

FULLY AUTOMATED LATEX IMMUNOASSAY; LIPOPROTEIN(A); ENZYME-LINKED IMMUNOSORBENT ASSAY; ISOFORMS; IMMUNE-CHEMISTRY ANALYZER 501X;

D O I：

10.1016/0009-8981(94)90038-8

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

We have developed a fully automated system to quantify lipoprotein(a) (Lp(a)) in human serum, based on the latex-enhanced turbidimetric immunoassay by application of the Immune Chemistry Analyzer 501X. This assay was carried out with undiluted serum and was able to detect at Lp(a) levels higher than 4.0 mg/l. When judged to be out of range of the calibration (> 600 mg/l), the sample was automatically re-tested after automatic 10-fold dilution. Within-run C.V.s ranged from 1.9 to 2.1% and between-run C.V.s from 2.7 to 3.9%. Results by the present method were in good agreement with those by the in-house ELISA (r = 0.978) and the commercial ELISA (r = 0.990). The distribution of Lp(a) levels in sera from 508 healthy donors was highly skewed; the mean and median were 158 mg/l and 105 mg/l, respectively.

引用

页码：105 / 113

页数：9

共 25 条

[1] STUDIES ON APOLIPOPROTEIN(A) PHENOTYPES .1. PHENOTYPE FREQUENCIES IN A HEALTHY JAPANESE POPULATION [J].