GENE-EXPRESSION IN RESPONSE TO MULTIPLE NUTRIENT-STARVATION CONDITIONS IN SALMONELLA-TYPHIMURIUM

被引:17
作者
SPECTOR, MP
机构
[1] Department of Biomedical Sciences, College of Allied Health Professions, University of South Alabama, Mobile, AL
基金
美国国家卫生研究院;
关键词
GLOBAL REGULATION CONTROL; PHOSPHATE LIMITATION; CARBON ENERGY SOURCE LIMITATION; NITROGEN LIMITATION; NAD LIMITATION; MU-D-LAC GENE FUSIONS;
D O I
10.1016/0378-1097(90)90552-2
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In nature, bacteria encounter a variety of environmental conditions, among the most frequent of these is the limitation or starvation of one or more essential nutrients. It is reasonable to assume, therefore, that bacteria have evolved mechanisms to enhance their survival over prolonged periods of nutrient starvation. We have identified eight genetic loci in the enteropathogen Salmonella typhimurium, using Mu d-directed lacZ operon fusion technology, that were induced in response to two or more different starvation conditions (sti). In simultaneous studies, we also identified genetic loci, using Mu d-lac fusion, which respond to only phosphate-starvation conditions (psi). We further characterized these loci as to their induction-characteristics, kinetics of induction, expression during growth on different carbon sources, and approximate location on the S. typhimurium genetic map. In concurrent studies, we analyzed whole cell extracts of S. typhimurium grown under a variety of nutrient starvation conditions as well as under nonlimiting conditions, using two-dimensional polyacrylamide gel electrophoresis. Results from these studies correlated well with our gene fusion studies. In more recent studies, we have demonstrated a complex genetic regulation of a number of these starvation-inducible loci, and have implicated at least four of these loci in the long term starvation-survival of S. typhimurium.
引用
收藏
页码:175 / 183
页数:9
相关论文
共 26 条
[1]   TRANSPOSITION AND FUSION OF LAC GENES TO SELECTED PROMOTERS IN ESCHERICHIA-COLI USING BACTERIOPHAGE-LAMBDA AND BACTERIOPHAGE-MU [J].
CASADABAN, MJ .
JOURNAL OF MOLECULAR BIOLOGY, 1976, 104 (03) :541-555
[2]  
CASADABAN MJ, 1979, P NATL ACAD SCI USA, V76, P4530, DOI 10.1073/pnas.76.9.4530
[3]  
DAVIS RW, 1980, MANUAL GENETIC ENG A
[4]   PHOSPHATE STARVATION REGULON OF SALMONELLA-TYPHIMURIUM [J].
FOSTER, JW ;
SPECTOR, MP .
JOURNAL OF BACTERIOLOGY, 1986, 166 (02) :666-669
[6]   STARVATION PROTEINS IN ESCHERICHIA-COLI - KINETICS OF SYNTHESIS AND ROLE IN STARVATION SURVIVAL [J].
GROAT, RG ;
SCHULTZ, JE ;
ZYCHLINSKY, E ;
BOCKMAN, A ;
MATIN, A .
JOURNAL OF BACTERIOLOGY, 1986, 168 (02) :486-493
[7]   PHYSIOLOGICAL-RESPONSES TO NUTRIENT LIMITATION [J].
HARDER, W ;
DIJKHUIZEN, L .
ANNUAL REVIEW OF MICROBIOLOGY, 1983, 37 :1-23
[8]  
HOLLEY EA, 1982, J BACTERIOL, V152, P959
[9]   STARVATION-INDUCED CROSS PROTECTION AGAINST HEAT OR H2O2 CHALLENGE IN ESCHERICHIA-COLI [J].
JENKINS, DE ;
SCHULTZ, JE ;
MATIN, A .
JOURNAL OF BACTERIOLOGY, 1988, 170 (09) :3910-3914
[10]   THE TRANSIENT PHASE BETWEEN GROWTH AND NONGROWTH OF HETEROTROPHIC BACTERIA, WITH EMPHASIS ON THE MARINE-ENVIRONMENT [J].
KJELLEBERG, S ;
HERMANSSON, M ;
MARDEN, P .
ANNUAL REVIEW OF MICROBIOLOGY, 1987, 41 :25-49