THE GENE FOR HYPOXANTHINE PHOSPHORIBOSYL TRANSFERASE OF PLASMODIUM-FALCIPARUM COMPLEMENTS A BACTERIAL HPT MUTATION

被引：13

作者：

SHAHABUDDIN, M ^{[1
]}

SCAIFE, J ^{[1
]}

机构：

[1] UNIV EDINBURGH,DEPT MOLEC BIOL,KINGS BLDG,MAYFIELD RD,EDINBURGH EH9 3JR,MIDLOTHIAN,SCOTLAND

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1990年 / 41卷 / 02期

关键词：

Complementation; Hypoxanthine phosphoribosyl transferase; Malaria; Plasmodium; Purine metabolism;

D O I：

10.1016/0166-6851(90)90191-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The enzyme hypoxanthine phosphoribosyl transferase of Plasmodium falciparum has been overexpressed in Escherichia coli. The protein was found to be active enzymatically. When the recombinant expression vector (pPfPRT2) was transformed and expressed in a Salmonella typhimurium mutant KP1684 (purE deoD hpt gpt), the active expressed protein complemented the hpt mutation in the bacteria. We discuss the practical value of this strain. Assays of the expressed protein in the mutant extract showed that the enzyme is able to use hypoxanthine, guanine and xanthine as substrates. A specificity study using the competitive inhibitor, 6-thioguanine, showed that of these hypoxanthine is the most favourable substrate. The biological significance of xanthine utilisation by the enzyme is discussed. © 1990.

引用

页码：281 / 288

页数：8

共 19 条

[1]

APPLEYARD RK, 1954, GENETICS, V39, P440

[2]

ARGOS P, 1983, J BIOL CHEM, V258, P6450

[3]

BULLAS LR, 1983, J BACTERIOL, V156, P471, DOI 10.1128/JB.156.1.471-474.1983

[4] NUCLEOTIDE-SEQUENCE AND ORGANIZATION OF THE MOUSE ADENINE PHOSPHORIBOSYLTRANSFERASE GENE - PRESENCE OF A CODING REGION COMMON TO ANIMAL AND BACTERIAL PHOSPHORIBOSYLTRANSFERASES THAT HAS A VARIABLE INTRON EXON ARRANGEMENT [J].

DUSH, MK ;

SIKELA, JM ;

KHAN, SA ;

TISCHFIELD, JA ;

STAMBROOK, PJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (09) :2731-2735

[5]

Gilmour D., 1965, METABOLISM INSECTS M

[6] ROLE OF HYPOXANTHINE AND GUANINE IN REGULATION OF SALMONELLA-TYPHIMURIUM-PUR GENE-EXPRESSION [J].

HOULBERG, U ;

JENSEN, KF .

JOURNAL OF BACTERIOLOGY, 1983, 153 (02) :837-845

[7] CHARACTERIZATION OF CDNA CLONES FOR HYPOXANTHINE GUANINE PHOSPHORIBOSYLTRANSFERASE FROM THE HUMAN MALARIAL PARASITE, PLASMODIUM-FALCIPARUM - COMPARISONS TO THE MAMMALIAN GENE AND PROTEIN [J].

KING, A ;

MELTON, DW .

NUCLEIC ACIDS RESEARCH, 1987, 15 (24) :10469-10481

[8]

KONIGK E, 1977, B WORLD HEALTH ORGAN, V55, P249

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10]

Maniatis T., 1982, MOL CLONING

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