A 5 KDA PROTEIN (SCS23) FROM THE 30-S SUBUNIT OF THE SPINACH CHLOROPLAST RIBOSOME

被引：11

作者：

WADA, A ^{[1
]}

KOYAMA, K ^{[1
]}

MAKI, Y ^{[1
]}

SHIMOI, Y ^{[1
]}

TANAKA, A ^{[1
]}

TSUJI, H ^{[1
]}

机构：

[1] KYOTO UNIV, FAC SCI, DEPT BOT, SAKYO KU, KYOTO 60601, JAPAN

来源：

FEBS LETTERS | 1993年 / 319卷 / 1-2期

关键词：

CHLOROPLAST RIBOSOME; RIBOSOMAL PROTEIN; SPINACH CHLOROPLAST;

D O I：

10.1016/0014-5793(93)80048-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The proteins of the 30 S ribosomal subunits from spinach chloroplasts were investigated using a radical-free and highly reducing (RFHR) method of two-dimensional polyacrylamide gel electrophoresis (PAGE). Twenty-three proteins were resolved on the gel down to the smallest protein of 5 kDa. The N-terminal amino acid sequence of the 5 kDa protein showed no homology with that of any other protein stored in databases, and the copy numbers were estimated to be 0.88 +/- 0.16 and 0.72 +/- 0.04 in the 30 S subunits and the 70 S ribosomes, respectively. The results suggest that the 5 kDa protein, which we have called SCS23, may be an essential ribosomal protein specific to spinach chloroplasts.

引用

页码：115 / 118

页数：4

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