THE LARGE LATE VIRUS TRANSCRIPTS SYNTHESIZED IN HERPESVIRUS SUIS (PSEUDORABIES) VIRUS-INFECTED CELLS ARE NOT PRECURSORS OF MESSENGER-RNA

The sixes of early and late pseudorabies virus transcripts were compared to those of early and late mRNA. While the early primary transcripts were of approximately the same size as early mRNA, a large proportion of late primary transcripts was much larger than late mRNA. Most early transcripts were transported efficiently to the cytoplasm and were relatively stable. A large proportion of the late transcripts were retained in the nucleus and turned over rapidly. Specific retention in the nucleus of transcripts originating from some regions of the genome could be detected. These were not preferentially degraded; degradation of transcripts originating from all regions of the genome, including those from which late mRNA originates, occurred. Experiments designed to determine whether part of the large transcripts are processed into mRNA revealed that most of the large late transcripts synthesized by the infected cells bear no precursor relationship to mRNA. Thus, during late phase of infection, most regions of the genome are abundantly transcribed as large RNA molecules; these are not destined to be processed into mRNA. [Rabbit kidney cells were used in this study.].