PURIFICATION AND CHARACTERIZATION OF TOXIN-B FROM A STRAIN OF CLOSTRIDIUM-DIFFICILE THAT DOES NOT PRODUCE TOXIN-A

被引:52
作者
TORRES, JF
机构
[1] GOTHENBURG UNIV,DEPT MED MICROBIOL,S-41346 GOTHENBURG,SWEDEN
[2] INST MEXICANO SEGURO SOCIAL,UNIDAD INVEST ENFERMEDADES INFECCIOSAS & PARASITARIAS,MEXICO CITY 7,DF,MEXICO
关键词
D O I
10.1099/00222615-35-1-40
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Most toxigenic strains of Clostridium difficile produce both toxin A and toxin B. The toxin produced by C. difficile strain 8864 was characterised and compared with those produced by C. difficile strain 10463. Toxin A was not detected by immunoassay in cultures from strain 8864 and all the cytotoxic activity produced by this strain was neutralised by antiserum to toxin B. Toxin B from strain 8864 was purified and compared with toxin B from strain 10463. The size of the purified subunits of toxin B from strain 8864 differed slightly from those of strain 10463 and there were small immunological differences. The effect on fibroblast cells was more like that of C. sordellii cytotoxin than of toxin B from strain 10463. These results suggest that C. difficile strain 8864 produces a modified toxin B and does not produce toxin A.
引用
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页码:40 / 44
页数:5
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