HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF OFLOXAXIN IN SERUM

A high-performance liquid chromatographic method was developed for the assay of ofloxacin in serum. The serum samples were filtered through a Molcut II membrane filter after partial deproteinization in order to remove proteins. The filtrate was preseparated on a reversed-phase precolumn and introduced onto a reversed-phase analytical column by applying column-switching techniques. Ofloxacin and nalidixic acid as internal standards were detected by absorbance measurements at 300 nm. Using this system the concentration of ofloxacin could be determined at a limit of the order of 20 ng/ml, with a linear dynamic range of 80 - 4000 ng/ml. This procedure was applied to pharmacokinetic studies of healthy volunteers treated with ofloxacin.