A COATED WIRE ELECTRODE SENSITIVE TO TETRAPHENYLPHOSPHONIUM ION FOR MEASUREMENT OF THE MITOCHONDRIAL-MEMBRANE POTENTIAL

被引：9

作者：

SATAKE, H

HORI, H

KANESHINA, S

机构：

[1] Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima, Minamijosanjima-cho

来源：

ANALYTICAL LETTERS | 1991年 / 24卷 / 02期

关键词：

COATED WIRE ELECTRODE; POTENTIOMETRY; TETRAPHENYLPHOSPHONIUM ION; MEMBRANE POTENTIAL; MITOCHONDRIA;

D O I：

10.1080/00032719108052905

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A coated wire electrode selectively sensitive to tetraphenylphosphonium(TPP+) ion has been developed, which employs TPP+-tetraphenylborate ion-pair as an ion sensor, dioctylphtalate as a plasticizer and poly(vinyl chloride) as an inert matrix. The electrode exhibits a linear response within the concentration range 10(-3) - 3x10(-7) M, with a Nernstian slope(56.5 mV/decade). The reproducibility, stability, response time and selectivity were also investigated. The electrode exhibits very good selectivity for TPP+ ion against the substances used for the measurement of membrane potential of mitochondria. The electrode was applied satisfactorily to the estimation of mitochondrial membrane potential.

引用

页码：295 / 304

页数：10

共 16 条

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