PLANT-CELL CULTURE USING A NOVEL BIOREACTOR - THE MAGNETICALLY STABILIZED FLUIDIZED-BED

被引：21

作者：

BRAMBLE, JL ^{[1
]}

GRAVES, DJ ^{[1
]}

BRODELIUS, P ^{[1
]}

机构：

[1] UNIV PENN,DEPT CHEM ENGN,PHILADELPHIA,PA 19104

来源：

BIOTECHNOLOGY PROGRESS | 1990年 / 6卷 / 06期

关键词：

D O I：

10.1021/bp00006a008

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel bioreactor using magnetically stabilized fluidized bed (MSFB) technology has been developed that has certain advantages for cultivating cells continuously. In this system, the cells are protected from shear and are constrained to move through the fermenter in lock-step fashion by being immobilized in calcium alginate beads. The MSFB permits good mass transfer, minimizes particle collisions, and allows for the production of cells while maintaining a controlled cell residence time. Details of the experimental system are described. In addition, the experimental performance of an MSFB used to grow plant cells in batch mode is compared to the results obtained in shake flask culture.

引用

页码：452 / 457

页数：6

共 20 条

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