RAPID AND SPECIFIC DETERMINATION OF SEROTONIN, ITS PRECURSORS AND METABOLITES IN RAT-BRAIN TISSUE BY LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION

被引:15
作者
TROUVIN, JH
GARDIER, A
GEMAYEL, GE
JACQUOT, C
机构
[1] Faculte de Pharmacie, Chatenay-Malabry, Fr, Faculte de Pharmacie, Chatenay-Malabry, Fr
来源
JOURNAL OF LIQUID CHROMATOGRAPHY | 1987年 / 10卷 / 01期
关键词
5-HYDROXYTRYPTOPHAN - BRAIN TISSUE - DOPAMINE - SEROTONIN;
D O I
10.1080/01483918708074196
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A liquid chromatographic analysis with electrochemical detection is proposed for simultaneous determination of four indols (i.e. tryptophan, 5-hydroxytryptophan, serotonin and 5-hydroxyindoleacetic acid) in various rat brain areas. Optimal elution conditions have been determined by studying chromatographic parameters: pH, buffer salt, counter-ion, organic modifier. Eventual interferences with the catecholaminergic compounds (i.e. norepinephrine, dopamine and their major metabolites) known to be detectable in the same conditions, have been eliminated. The specificity of the four indol detection was demonstrated by chromatographic, electrochemical and pharmacological proofs.
引用
收藏
页码:121 / 136
页数:16
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