TEMPORAL EFFECTS OF TAURINE ON MOUSE PREIMPLANTATION DEVELOPMENT INVITRO

被引：24

作者：

DUMOULIN, JCM

EVERS, JLH

BAKKER, JA

BRAS, M

PIETERS, MHEC

GERAEDTS, JPM

机构：

[1] Department of Obstetrics and Gynaecology, Academic Hospital Maastricht, University of Limburg

[2] Department of Cell Biology and Genetics, University of Limburg

来源：

HUMAN REPRODUCTION | 1992年 / 7卷 / 03期

关键词：

TAURINE; PREIMPLANTATION DEVELOPMENT; MOUSE EMBRYO; 2-CELL BLOCK;

D O I：

10.1093/oxfordjournals.humrep.a137658

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Previously it has been shown that significantly more 2-cell mouse embryos reach the blastocyst stage when cultured in medium supplemented with taurine. In this study, in-vitro fertilized zygotes from a hybrid mouse strain were used to examine the temporal effects of 10 mM taurine on embryonic development in vitro during the preimplantation period. Taurine exerted its beneficial effect exclusively during the first 2 days post-insemination. The effect of taurine on blastocyst formation appeared to be restricted mostly to the period 20-48 h after fertilization, during which time mouse embryos are at the two-cell stage. Although more blastocysts were found when embryos were cultured in taurine-containing medium from 5 to 20 h post-insemination, this difference was not significant compared to the number of blastocysts when embryos were cultured without taurine. Taurine did not appear to affect the two-cell block of mouse embryos from random-bred strains.

引用

页码：403 / 407

页数：5

共 29 条

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