BIOLUMINESCENT REPORTER BACTERIA DETECT CONTAMINANTS IN SOIL SAMPLES

被引：42

作者：

BURLAGE, RS ^{[1
]}

PALUMBO, AV ^{[1
]}

HEITZER, A ^{[1
]}

SAYLER, G ^{[1
]}

机构：

[1] UNIV TENNESSEE, CTR ENVIRONM BIOTECHNOL, KNOXVILLE, TN 37932 USA

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1994年 / 45-6卷

关键词：

NAPHTHALENE; TOLUENE; BIOLUMINESCENT; BIOREPORTER; BIOAVAILABILITY;

D O I：

10.1007/BF02941845

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Reporter strains of bacteria were tested using soil samples from several sites near a leaking fuel oil storage facility. The reporter bacteria utilized the bioluminescent lux genes from Vibrio fischeri, which were transcriptionally fused to catabolic gene sequences. The catabolic genes of interest specified the degradation of toluene (from the TOL plasmid) and naphthalene (from the NAH7 plasmid and from a NAH plasmid recently isolated). The results indicated that two soil samples were contaminated with both toluene (or xylene) and naphthalene. These data were useful in describing the extent of contamination at the site.

引用

页码：731 / 740

页数：10

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