XENOPUS PKN - CLONING AND SEQUENCING OF THE CDNA AND IDENTIFICATION OF CONSERVED DOMAINS

被引：28

作者：

MUKAI, H ^{[1
]}

MORI, K ^{[1
]}

TAKANAGA, H ^{[1
]}

KITAGAWA, M ^{[1
]}

SHIBATA, H ^{[1
]}

SHIMAKAWA, M ^{[1
]}

MIYAHARA, M ^{[1
]}

ONO, Y ^{[1
]}

机构：

[1] KOBE UNIV, FAC SCI, DEPT BIOL, KOBE 657, JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1995年 / 1261卷 / 02期

关键词：

PROTEIN KINASE; CDNA SEQUENCE; GENE EXPRESSION; (X-LAEVIS);

D O I：

10.1016/0167-4781(95)00030-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

cDNA clone encoding Xenopus laevis PKN has been isolated from Xenopus kidney library. Sequencing of this clone has revealed a single open reading frame encoding a protein of 901 amino acids. Immunoprecipitate from cytoplasmic fraction of COS7 cells transfected with this cDNA construct using antiserum against bacterially expressed Xenopus PKN revealed arachidonic acid-dependent autophosphorylation activity. Comparison of the closely related sequences of human and rat PKN with a protein from evolutionarily distant Xenopus, revealed several highly invariant domains in the NH2-terminal regulatory regions, suggesting that they participate in binding interaction with arachidonic acid.

引用

页码：296 / 300

页数：5

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