ENDOTHELIAL-CELL DIFFERENTIATION INTO CAPILLARY-LIKE STRUCTURES IN RESPONSE TO TUMOR-CELL CONDITIONED MEDIUM - A MODIFIED CHEMOTAXIS CHAMBER ASSAY

被引：32

作者：

GARRIDO, T ^{[1
]}

RIESE, HH ^{[1
]}

ARACIL, M ^{[1
]}

PEREZARANDA, A ^{[1
]}

机构：

[1] PHARM ANTIBIOT FARMA SA,RES DEPT,E-28026 MADRID,SPAIN

来源：

BRITISH JOURNAL OF CANCER | 1995年 / 71卷 / 04期

关键词：

ANGIOGENESIS; CHEMOTAXIS CHAMBER; BOVINE AORTIC ENDOTHELIAL CELLS; MATRIGEL; BASIC FIBROBLAST GROWTH FACTOR;

D O I：

10.1038/bjc.1995.149

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We have developed a modified chemotaxis chamber assay in which bovine aortic endothelial (BAE) cells degrade Matrigel basement membrane and migrate and form capillary-like structures on type I collagen. This capillary formation occurs in the presence of conditioned media from highly metaslatic tumour cell lines, such as B16F10 murine melanoma or MDA-MB-231 human breast adenocarcinoma, but not in the presence of conditioned medium (CM) from the less invasive B16F0 cell line. Replacement of tumour cell CM by 10 ng ml(-1) basic fibroblast growth factor (bFGF) also results in capillary-like structure formation by BAE cells. An anti-bFGF antibody blocks this effect, showing that bFGF is one of the factors responsible for the angiogenic response induced by B16F10 CM in our assay. Addition of an anti-laminin antibody reduces significantly the formation of capillary-like structures, probably by blocking the attachment of BAE cells to laminin present in Matrigel. The anti-angiogenic compound suramin inhibits in a dose-dependent manner (complete inhibition with 100 mu M suramin) the migration and differentiation of BAE cells on type I collagen in response to B16F10 CM. This assay represents a new model system to study tumour-induced angiogenesis in vitro.

引用

页码：770 / 775

页数：6

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