MORPHOLOGICAL FEATURES OF CLONED LYMPHOCYTES EXPRESSING GAMMA-DELTA T-CELL RECEPTORS

被引:16
作者
ARANCIA, G
MALORNI, W
IOSI, F
ZARCONE, D
CERRUTI, G
FAVRE, A
ZEROMSKI, J
GROSSI, CE
MORETTA, A
机构
[1] IST SUPER SANITA, ULTRASTRUTTURE LAB, I-00161 ROME, ITALY
[2] UNIV GENOA, IST ANAT & ISTOL, I-16132 GENOA, ITALY
[3] IST NAZL RIC CANC, GENOA, ITALY
关键词
D O I
10.1002/eji.1830210126
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We have analyzed the morphological characteristics of human T lymphocytes bearing CD3-associated T cell receptor (TcR)-gamma and delta-chains. BB3 and delta-TCS1 monoclonal antibodies (mAb) were used to identify two distinct, nonoverlapping populations of TcR-gamma/delta+ cells which express the products of V-delta-2 and V-delta-1 gene segments, respectively. In the peripheral blood, most V-delta-1+ (delta-TCS-1+) lymphocytes express the non-disulfide-linked form of receptor whereas V-delta-2+ (BB3+) cells express the disulfide-linked form. The majority of cloned TcR-gamma/delta+ cells exhibit a growth pattern different from that of conventional TcR-alpha/beta+ cells as they adhere promptly to surfaces and undergo morphological changes which can be summarized as follows: cells spread on the surface, form a distinct uropod and, in the final phase of adherence, emit long filopodia ending with adhesion plaques. Immunofluorescence studies of TcR-gamma/delta+ clones demonstrated the presence of submembranous actin microfilaments and actin-binding protein confirming that these cells are capable of active motility which is related to the propensity of TcR-gamma/delta+ cells to home to epithelia. Scanning electron microscope analyses of effector/target cell conjugates showed that in TcR-gamma/delta+ cells the region of the uropodia next to the cell body is responsible for the binding to tumor target cells. Interestingly, immunofluorescence analyses revealed that LFA-1 molecules are predominantly distributed in the uropodium whereas they are virtually absent in the cell bodies. These morphological characteristics of TcR-gamma/delta+ cells may pertain to defensive mechanisms at the mucosal level.
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页码:173 / 178
页数:6
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