IMMOBILIZATION OF CATALASE ON MEMBRANES OF POLY(ETHYLENE)-G-CO-ACRYLIC ACID AND POLY(TETRAFLUOROETHYLENE)-G-CO-ACRYLIC ACID AND THEIR APPLICATION IN HYDROGEN-PEROXIDE ELECTROCHEMICAL SENSORS

被引：16

作者：

DASILVA, MA ^{[1
]}

GIL, MH ^{[1
]}

PIEDADE, AP ^{[1
]}

REDINHA, JS ^{[1
]}

BRETT, AMO ^{[1
]}

COSTA, JMC ^{[1
]}

机构：

[1] UNIV COIMBRA,DEPT QUIM,P-3049 COIMBRA,PORTUGAL

来源：

JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY | 1991年 / 29卷 / 02期

关键词：

D O I：

10.1002/pola.1991.080290215

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

The immobilization of catalase on grafted membranes of poly(ethylene)-g-co-acrylic acid and poly(tetrafluoroethylene)-g-co-acrylic acid and their application in hydrogen peroxide electrochemical sensors is described. The introduction of carboxylic acid groups onto a hydrophobic support provides a good environment for subsequent enzyme immobilization. This single membrane, hydrogen peroxide sensor showed significant improvement with respect to the double membrane versions. The response is very rapid, the linear range being from 10-mu-M up to 6 mM, with a detection limit of 4.7-mu-M, and a lifetime of more than 4 months.

引用

页码：269 / 274

页数：6

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