EVALUATION OF MALONALDEHYDE AS A MARKER OF OXIDATIVE RANCIDITY IN MEAT-PRODUCTS

被引:76
作者
SHAHIDI, F
HONG, C
机构
[1] Department of Biochemistry Memorial, University of Newfoundland, St. John'S, Newfoundland
关键词
D O I
10.1111/j.1745-4514.1991.tb00147.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Quantification of malonaldehyde as a marker of lipid oxidation in comminuted meat systems, with or without additives, by a distillation and an extraction procedure was investigated. Each method had its own benefits and drawbacks. The extraction procedure generally afforded more realistic results. However, presence of colored additives such as the cooked cured-meat pigment, as well as dispersed lipids in the extracts, interfered with accurate determination of the colored chromogen between malonaldehyde and the 2-thiobarbituric acid (TBA) reagent. On the other hand, the distillation procedure afforded higher TBA values as compared to the extraction method since decomposition of labile hydroperoxides occurred. Addition of antioxidants to the mixtures prior to distillation proved beneficial in some cases. In the presence of residual nitrite, addition of sulfanilamide to the mixture prior to distillation prevented underestimation of malonaldehyde presumably by inhibiting its nitrosation.
引用
收藏
页码:97 / 105
页数:9
相关论文
共 20 条
[1]  
Cochran W.G, 1957, STAT METHODS, V6th ed
[2]   DETERMINATION OF TBA NUMBER BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
KAKUDA, Y ;
STANLEY, DW ;
VANDEVOORT, FR .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1981, 58 (07) :773-775
[3]  
Kohn HI, 1944, J PHARMACOL EXP THER, V82, P292
[4]   STUDIES ON TBA TEST FOR RANCIDITY GRADING - .2. TBA REACTIVITY OF DIFFERENT ALDEHYDE CLASSES [J].
MARCUSE, R ;
JOHANSSON, L .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1973, 50 (10) :387-391
[5]   THE THIOBARBITURIC ACID TEST FOR LIPID-PEROXIDATION - STRUCTURE OF THE ADDUCT WITH MALONDIALDEHYDE [J].
NAIR, V ;
TURNER, GA .
LIPIDS, 1984, 19 (10) :804-805
[6]   ASSAY FOR LIPID PEROXIDES IN ANIMAL-TISSUES BY THIOBARBITURIC ACID REACTION [J].
OHKAWA, H ;
OHISHI, N ;
YAGI, K .
ANALYTICAL BIOCHEMISTRY, 1979, 95 (02) :351-358
[7]   ESTIMATION OF PRODUCT OF LIPID PEROZIDATION (MALONYL DIALDEHYDE) IN BIOCHEMICAL SYSTEMS [J].
PLACER, ZA ;
CUSHMAN, LL ;
JOHNSON, BC .
ANALYTICAL BIOCHEMISTRY, 1966, 16 (02) :359-&
[8]   CONTROL OF LIPID OXIDATION IN COOKED GROUND PORK WITH ANTIOXIDANTS AND DINITROSYL FERROHEMOCHROME [J].
SHAHIDI, F ;
RUBIN, LJ ;
WOOD, DF .
JOURNAL OF FOOD SCIENCE, 1987, 52 (03) :564-567
[9]  
Shahidi F., 1991, Journal of Muscle Foods, V2, P1, DOI 10.1111/j.1745-4573.1991.tb00436.x
[10]  
SHAHIDI F, 1988, 34TH P INT C MEAT SC, P357