ROLES OF SALMONELLA-TYPHIMURIUM UMUDC AND SAMAB IN UV MUTAGENESIS AND UV SENSITIVITY

被引:30
作者
NOHMI, T [1 ]
YAMADA, M [1 ]
WATANABE, M [1 ]
MURAYAMA, SY [1 ]
SOFUNI, T [1 ]
机构
[1] TEIKYO UNIV,SCH MED,DEPT BACTERIOL,ITABASHI KU,TOKYO 173,JAPAN
关键词
D O I
10.1128/JB.174.21.6948-6955.1992
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Expression of the umuDC operon is required for UV mutagenesis and most chemical mutagenesis in Escherichia coli. The closely related species Salmonella typhimurium has two sets of umuDC-like operons; the samAB operon is located in a 60-MDa cryptic plasmid, while the S. typhimurium umuDC (umuDC(ST)) operon resides in a chromosome. The roles of these two umuDC-like operons in UV mutagenesis and UV sensitivity of S. typhimurium were investigated. A pBR322-derived plasmid carrying the samAB operon more efficiently restored UV mutability to a umuD44 strain and a umuC122::Tn5 strain of E. coli than a plasmid carrying the umuDC(ST) operon did. When the umuDC(ST) operon was specifically deleted from the chromosome of S. typhimurium TA2659, the resulting strain was not UV mutable and was more sensitive to the killing effect of UV irradiation than the parent strain was. Curing of the 60-MDa cryptic plasmid carrying the samAB operon did not influence the UV mutability of strain TA2659 but did increase its resistance to UV killing. A pSC101-derived plasmid carrying the samAB operon did not restore UV mutability to a umuD44 strain of E. coli, whereas pBR322- or pBluescript-derived plasmids carrying the samAB operon efficiently did restore UV mutability. We concluded that the umuDC(ST) operon plays a major role in UV mutagenesis in S. typhimurium and that the ability of the samAB operon to promote UV mutagenesis is strongly affected by gene dosage. Possible reasons for the poor ability of samAB to promote UV mutagenesis when it is present on low-copy-number plasmids are discussed.
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页码:6948 / 6955
页数:8
相关论文
共 52 条
[1]   INDUCIBILITY OF A GENE-PRODUCT REQUIRED FOR UV AND CHEMICAL MUTAGENESIS IN ESCHERICHIA-COLI [J].
BAGG, A ;
KENYON, CJ ;
WALKER, GC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (09) :5749-5753
[2]   A RECA PROTEIN MUTANT DEFICIENT IN ITS INTERACTION WITH THE UMUDC COMPLEX [J].
BAILONE, A ;
SOMMER, S ;
KNEZEVIC, J ;
DUTREIX, M ;
DEVORET, R .
BIOCHIMIE, 1991, 73 (04) :479-484
[3]   DOMINANT NEGATIVE UMUD MUTATIONS DECREASING RECA-MEDIATED CLEAVAGE SUGGEST ROLES FOR INTACT UMUD IN MODULATION OF SOS MUTAGENESIS [J].
BATTISTA, JR ;
OHTA, T ;
NOHMI, T ;
SUN, W ;
WALKER, GC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (18) :7190-7194
[4]  
BATTISTA JR, 1988, MECHANISMS CONSEQUEN, P455
[5]  
BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513
[7]   UMUD MUTAGENESIS PROTEIN OF ESCHERICHIA-COLI - OVERPRODUCTION, PURIFICATION, AND CLEAVAGE BY RECA [J].
BURCKHARDT, SE ;
WOODGATE, R ;
SCHEUERMANN, RH ;
ECHOLS, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (06) :1811-1815
[8]   GROE MUTANTS OF ESCHERICHIA-COLI ARE DEFECTIVE IN UMUDC-DEPENDENT UV MUTAGENESIS [J].
DONNELLY, CE ;
WALKER, GC .
JOURNAL OF BACTERIOLOGY, 1989, 171 (11) :6117-6125
[9]   COEXPRESSION OF UMUD' WITH UMUC SUPPRESSES THE UV MUTAGENESIS DEFICIENCY OF GROE MUTANTS [J].
DONNELLY, CE ;
WALKER, GC .
JOURNAL OF BACTERIOLOGY, 1992, 174 (10) :3133-3139
[10]   NEW RECA MUTATIONS THAT DISSOCIATE THE VARIOUS RECA PROTEIN ACTIVITIES IN ESCHERICHIA-COLI PROVIDE EVIDENCE FOR AN ADDITIONAL ROLE FOR RECA PROTEIN IN UV MUTAGENESIS [J].
DUTREIX, M ;
MOREAU, PL ;
BAILONE, A ;
GALIBERT, F ;
BATTISTA, JR ;
WALKER, GC ;
DEVORET, R .
JOURNAL OF BACTERIOLOGY, 1989, 171 (05) :2415-2423