CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF FRUCTOSE 6-PHOSPHATE, 2-KINASE-FRUCTOSE 2,6-BISPHOSPHATASE

被引：7

作者：

ISTVAN, ES

HASEMANN, CA

KURUMBAIL, RG

UYEDA, K

DEISENHOFER, J

机构：

[1] UNIV TEXAS,SW MED CTR,HOWARD HUGHES MED INST,DALLAS,TX 75235

[2] UNIV TEXAS,SW MED CTR,DEPT BIOCHEM,DALLAS,TX 75235

[3] DEPT VET AFFAIRS MED CTR,RES SERV,DALLAS,TX 75216

来源：

PROTEIN SCIENCE | 1995年 / 4卷 / 11期

关键词：

BIFUNCTIONAL ENZYME; ENZYME MECHANISM; KINASE; PHOSPHATASE GLYCOLYSIS REGULATION;

D O I：

10.1002/pro.5560041125

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Diffraction-quality crystals of the bifunctional enzyme fructose 6-phosphate, 2-kinase:fructose 2,6-bisphosphatase from rat testis have been obtained. The crystals were grown in the presence of ATP gamma S, fructose 6-phosphate, the detergent n-octylglucoside, and the precipitant polyethylene glycol 4000. The crystals have the symmetry of the trigonal space group P3(1/2)21 with a = b = 83.0 Angstrom and c = 130.6 Angstrom. Flash-frozen crystals diffract to beyond 2.2 Angstrom, and native data have been collected.

引用

页码：2439 / 2441

页数：3

共 21 条

[1]

ALGAIER J, 1988, BIOCHEM BIOPH RES CO, V263, P839

[2]

CREPIN KM, 1992, J BIOL CHEM, V267, P21698

[3] COMPLETE NUCLEOTIDE-SEQUENCE CODING FOR RAT-LIVER 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE DERIVED FROM A CDNA CLONE [J].