EVIDENCE FOR A GLYCYL-PROLINE TRANSPORT-SYSTEM IN OVINE ENTEROCYTE BRUSH-BORDER MEMBRANE-VESICLES

被引：10

作者：

BACKWELL, FRC ^{[1
]}

WILSON, D ^{[1
]}

SCHWEIZER, A ^{[1
]}

机构：

[1] UNIV HOHENHEIM,INST BIOL CHEM & NUTR,D-70593 STUTTGART,GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 215卷 / 02期

关键词：

D O I：

10.1006/bbrc.1995.2501

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A peptide transport system in ovine enterocyte BBMV has been examined with glycyl-L-proline (Gly-Pro) as substrate. Transport is stimulated by an inwardly directed proton gradient (pH(i) = 8.4; pH(o) = 6.0). Gly-Pro uptake was saturable and conformed to Michaelis-Menten kinetics. Uptake was not affected by the presence of glycine but was inhibited by glycyl-L-phenylalanine (Gly-Phe). This is thought to be the first positive demonstration of the existence of a peptide transporter in the ruminant small intestine. (C) 1995 Academic Press, Inc.

引用

页码：561 / 565

页数：5

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