EXPERIENCE WITH A SIMPLE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY METHOD FOR THE ANALYSIS OF PURINE AND PYRIMIDINE NUCLEOSIDES AND BASES IN BIOLOGICAL-FLUIDS

被引：16

作者：

BENNETT, MJ ^{[1
]}

CARPENTER, KH ^{[1
]}

机构：

[1] CHILDRENS HOSP, DEPT CHEM PATHOL, SHEFFIELD S10 2TH, S YORKSHIRE, ENGLAND

来源：

ANNALS OF CLINICAL BIOCHEMISTRY | 1984年 / 21卷 / MAR期

关键词：

D O I：

10.1177/000456328402100210

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Purine and pyrimidine nucleosides and bases were analyzed using an isocratic reverse-phase HPLC [high performance liquid chromatography] technique and utilizing sample preparation. The method was successfully used to separate a large number of biologically occurring nucleic acid products within a 30-min period and to identify them by their chromatographic and optical properties. A number of [human] metabolic disorders were identified, including hypoxanthine-guanine phosphoribosyl transferase and ornithine carbamyl transferase deficiencies. The method has been in routine use for 2 yr for performing both quantitative and qualitative analysis and has proved to be robust and reliable.

引用

页码：131 / 136

页数：6

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