CLONING OF A PROBE FOR A PREVIOUSLY UNDESCRIBED ENTEROBACTERIAL TETRACYCLINE RESISTANCE GENE

被引:7
作者
JONES, CS [1 ]
OSBORNE, DJ [1 ]
STANLEY, J [1 ]
机构
[1] CENT PUBL HLTH LAB,NATL COLLECT TYPE CULTURES,MOLEC GENET UNIT,61 COLINDALE AVE,LONDON NW9 5HT,ENGLAND
关键词
D O I
10.1111/j.1472-765X.1992.tb00738.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
An 8.35 kb BamHI fragment was cloned from the plasmid R714a. It encoded resistances to chloramphenicol, streptomycin, spectinomycin and tetracycline. Tetracycline resistance was determined by a locus without homology to the known enterobacterial gene classes, TetA-TetE. Further subcloning of the fragment located an unusual tetracycline resistance gene on a 4.7 kb BamHI-Bg/II fragment. A physical-genetic map of this fragment indicated that the gene was bisected by a PstI site. Deletion analysis and insertion mutagenesis were used to define a suitable probe. An intragenic PstI-AvaI fragment of 1.2 kb was identified, and used as a non-radioactive probe, being specific for this previously undescribed enterobacterial Tet gene.
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页码:106 / 108
页数:3
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