EPITOPES ON THYROGLOBULIN - A STUDY OF PATIENTS WITH THYROID-DISEASE

被引：41

作者：

CATUREGLI, P ^{[1
]}

MARIOTTI, S ^{[1
]}

KUPPERS, RC ^{[1
]}

BUREK, CL ^{[1
]}

PINCHERA, A ^{[1
]}

ROSE, NR ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DEPT IMMUNOL & INFECT DIS,BALTIMORE,MD 21205

来源：

AUTOIMMUNITY | 1994年 / 18卷 / 01期

关键词：

THYROGLOBULIN; ANTIBODIES; AUTOIMMUNE THYROID DISEASES; THYROID CARCINOMA; NONTOXIC GOITER;

D O I：

10.3109/08916939409014678

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Thyroglobulin antibodies (TgAbs) are typically found in autoimmune thyroid diseases and, more rarely, in nonautoimmune thyroid diseases and healthy subjects. To determine whether TgAbs associated with different conditions recognize different epitopes on the thyroglobulin molecule, we studied 28 patients with Hashimoto's thyroiditis, 30 with Graves' disease, 21 with thyroid carcinoma, 18 with nontoxic goiter, and 2 5 healthy subjects. All patients were selected for the presence of TgAbs; 4/25 healthy subjects also had TpAbs. The sera were assayed for the their ability to inhibit the binding of monoclonal antibodies to thyroglobulin in an ELISA assay. We found that: 1) TgAbs in Hashimoto's patients preferentially recognized three clusters of epitopes (II, III and typically VI), with no difference between the goitrous and the atrophic variants; 2) TgAbs in Graves' patients were directed toward cluster II, with no difference between the presence or the absence of ophthalmopathy; 3) TgAbs in thyroid carcinoma patients recognized the same clusters as Hashimoto's patients; 4) TgAbs in nontoxic goiter patients and in the four healthy subjects showed no restriction in epitope recognition. We suggest that in individuals with no overt clinical or biochemical thyroid abnormalities but with TgAbs, the finding that these TgAbs recognize particular immunodominant clusters may be utilized to predict full-blown thyroid disorders. Longitudinal studies are needed to evaluate the possible clinical application of this methodology.

引用

页码：41 / 49

页数：9

共 27 条

[1] Weetman A.P., McGregor A.M., Autoimmune thyroid disease: developments in our understanding, End Rev, 5, pp. 309-355, (1984)
[2] DeGroot L.J., Hoyc K., Refetoff S., Scrum antigens and antibodies in the diagnosis of thyroid cancer, J Clin Endocrinol Metab, 45, pp. 1220-1223, (1980)
[3] Pacini R., Mariotti S., Formica N., Elisei R., Anelli S., Capotorti E., Pinchera A., Thyroid autoantibodies in thyroid cancer: incidence and relationship with tumour outcome, Acta Endocrinol, 119, pp. 373-380, (1988)
[4] Ericsson U.B., Christensen S.B., Thorcll J.I., A high prevalence of thyroglobulin autoantibodies in adults with and without thyroid disease as measured with a sensitive solid-phase immunosorbent radioassay, Clin Immunol Immunopathol, 37, pp. 154-162, (1985)
[5] Doullay F., Ruf J., Codaccioni J.L., Carayon P., Prevalence of autoantibodies to thyroidperoxidase in patients with various thyroid and autoimmune diseases, Autoimmunity, 9, pp. 237-244, (1991)
[6] Boyden S.V., Natural antibodies and the autoimmune responses, Adv Immunol, 5, pp. 1-28, (1965)
[7] Guilbert B., Dighicro G., Avrameas S., Naturally occurring antibodies against nine common antigens in human sera, J Immunol, 128, pp. 2779-2787, (1982)
[8] Kohno T., Tsunetoshi Y., Ishikawa E., Existence of anti-thyroglobulin IgG in healthy subjects, Biochem Biophys Res Commun, 155, pp. 224-229, (1988)
[9] Ruf J., Carayon P., Lissitzky S., Various expressions of a unique anti-human thyroglobulin antibody repertoire in normal state and autoimmune disease, Eur J Immunol, 15, pp. 268-272, (1985)
[10] Piechaczyk M., Bouanani M., Salhi S.L., Baldct L., Pau B., Bastide J.M., Antigenic domains on the human thyroglobulin molecule recognized by autoantibodies in patients' sera and by natural autoantibodies isolated from sera of healthy subjects, Clin Immunol Immunopathol, 45, pp. 114-121, (1987)

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