CLONING OF GENES INVOLVED IN NEGATIVE REGULATION OF PRODUCTION OF EXTRACELLULAR ENZYMES AND POLYSACCHARIDE OF XANTHOMONAS-CAMPESTRIS PATHOVAR CAMPESTRIS

被引:38
作者
TANG, JL [1 ]
GOUGH, CL [1 ]
DANIELS, MJ [1 ]
机构
[1] JOHN INNES INST,SAINSBURY LAB,COLNEY LANE,NORWICH NR4 7UH,NORFOLK,ENGLAND
来源
MOLECULAR & GENERAL GENETICS | 1990年 / 222卷 / 01期
关键词
Amylase; Endoglucanase; Pathogenicity; Polygalacturonate lyase; Protease;
D O I
10.1007/BF00283038
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A recombinant plasmid pIJ3079 contains DNA sequences from Xanthomonas campestris pv campestris involved in coordinate negative regulation of production of the extracellular enzymes protease, endoglucanase, amylase and polygalacturonate lyase, and extracellular polysaccharide (EPS). Wild-type bacteria harbouring pIJ3079 and therefore carrying extra copies of the gene(s) therein showed reduced enzyme and EPS production and reduced aggresiveness to plants. Localised Tn5 mutagenesis of the corresponding region of the genome gave mutants producing higher levels of enzymes and EPS than the wild type, suggesting that the gene(s) may negatively regulate production in the normal cell. Enzyme and EPS production in the mutants was still dependent on previously characterised positive regulatory genes. © 1990 Springer-Verlag.
引用
收藏
页码:157 / 160
页数:4
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