STUDIES ON THE PROTEINS INVOLVED IN THE INTERACTION OF HIGH-DENSITY-LIPOPROTEIN WITH ISOLATED HUMAN SMALL-INTESTINE EPITHELIAL-CELLS

被引：5

作者：

SVIRIDOV, DD ^{[1
]}

EHNHOLM, C ^{[1
]}

TENKANEN, H ^{[1
]}

PAVLOV, MY ^{[1
]}

SAFONOVA, IG ^{[1
]}

REPIN, VS ^{[1
]}

机构：

[1] NATL PUBL HLTH INST,SF-00300 HELSINKI,FINLAND

来源：

FEBS LETTERS | 1992年 / 303卷 / 2-3期

关键词：

APOLIPOPROTEIN-A-I; APOLIPOPROTEIN-A-II; HIGH-DENSITY LIPOPROTEIN RECEPTOR; HUMAN ENTEROCYTE;

D O I：

10.1016/0014-5793(92)80519-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Treatment of I-125-labelled high-density lipoprotein ([I-125]HDL3) with monospecific polyclonal antibodies against apolipoproteins A-I and A-II resulted in a dose-dependent inhibition of the [I-125]HDL3, binding to isolated human small intestine epithelial cells by 25% and 50%, respectively. Both antibodies also inhibited intracellular degradation of [I-125]HDL3 by 80%. Treatment of enterocytes with polyclonal antibody against apolipoprotein A-I binding protein, a putative HDL receptor, inhibited both binding and degradation of [I-125]HDL3 by these cells by 50%. Antibodies to apolipoprotein A-I, A-II and apo A-I-binding protein also inhibited [I-125]HDL3 binding to cholesterol-loaded cells.

引用

页码：202 / 204

页数：3

共 14 条

[1] THE INTERACTION OF HIGH-DENSITY-LIPOPROTEINS WITH EXTRAHEPATIC CELLS [J].