ACID PULLULANASE FROM BACILLUS-POLYMYXA MIR-23

被引：11

作者：

CASTRO, GR ^{[1
]}

SANTOPIETRO, LMD ^{[1
]}

SINERIZ, F ^{[1
]}

机构：

[1] UNIV BUENOS AIRES,FAC CIENCIAS EXACTAS & NAT,DEPT QUIM BIOL,MICROBIOL LAB,RA-1428 BUENOS AIRES,ARGENTINA

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1992年 / 37卷 / 03期

关键词：

BACILLUS-POLYMYXA; PULLULANASE; ALPHA-AMYLASE; ALPHA-GLUCOSIDASE; STARCH DEGRADATION; BATCH CULTURE; ACIDOPHILIC MICROORGANISM;

D O I：

10.1007/BF02788874

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Within the frame of a screening program aimed at the isolation of amylolytic sporeformers, one strain with high amylolytic activity designated MIR-23 was selected. The microbial characterization was carried out by morphological and biochemical tests and, by means of statistical treatment, was identified as Bacillus polymyxa. The organism could grow in acidic conditions (pH 5.0) on a starch medium and produce alpha-amylase, pullulanase, and alpha-glucosidase. Batch cultures showed the highest enzyme activities in the stationary phase. Pullulanase activity exhibited an optimal temperature of 52-57-degrees-C at pH 4.5-5.5. These properties would allow its use in the saccharification processes in the starch industries.

引用

页码：227 / 233

页数：7

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