ONTOGENY OF SOMATOSTATIN GENE-EXPRESSION IN RAT FOREBRAIN

With hybridization histochemistry, somatostatin (SRIF) mRNA was detected in several neuronal populations of the basal diencephalon (anterior and posterior) and basal telencephalon (lateral) for the first time on the 14th day of gestation (E14). On E16, a large increase of the extent of expression was found in these populations. In addition, cells in the medial telencephalon and a few cells in the future allocortex also contained SRIF mRNA for the first time. In the prenatal period, the expression in the above populations continued to mature and individual nuclei with SRIF mRNA began to be recognizable. At birth, the overall pattern of SRIF gene expression was established but the ventral portions (hypothalamus, amygdala, allocortical areas) had higher levels of expression than the more dorsal ones (striatum and neocortex). Over the first 2 wk of life, this difference decreased and an adult-like pattern was found at postnatal day 21. We demonstrate that most of SRIF gene expression development takes place before birth. This description may serve as a basis for studies on the putative functions of SRIF during brain ontogeny.