DISULFIDE BRIDGE STRUCTURE OF THE HEAT-STABLE SWEET PROTEIN MABINLIN-II

被引：21

作者：

NIRASAWA, S

LIU, XZ

NISHINO, T

KURIHARA, Y

机构：

[1] YOKOHAMA NATL UNIV, FAC EDUC, DEPT CHEM, HODOGAYA KU, YOKOHAMA, KANAGAWA 240, JAPAN

[2] YOKOHAMA CITY UNIV, SCH MED, DEPT BIOCHEM, YOKOHAMA, KANAGAWA 232, JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1202卷 / 02期

关键词：

DISULFIDE BRIDGE; SWEET PROTEIN; MABINLIN-II; HEAT STABILITY;

D O I：

10.1016/0167-4838(93)90016-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The heat-stable sweet protein mabinlin was composed of a A-chain of 33 amino-acid residues and a B-chain of 72 amino-acid residues (Liu, X., Maeda, S., Hu, Z., Aiuchi, T., Nakaya, K. and Kurihara, Y. (1993) Eur. J. Biochem. 211, 281-287). A-chain and B-chain contain two and six cysteine residues, respectively. The formation of two interchain disulfide bridges at Cys(A5)-Cys(B21) and Cys(A18)-Cys(B10), and two intrachain disulfide bridges at Cys(B11)-Cys(B59) and Cys(B23)-Cys(B67) were determined by amino-acid sequencing and composition analysis of cystine-containing peptides isolated by HPLC. Cleavage of the disulfide bridges with dithiothreitol results in complete loss of the sweet activity of mabinlin II. It was suggested that the structure fixed by four disulfide bridges contributes to heat stability of mabinlin II.

引用

页码：277 / 280

页数：4

共 19 条

[1] CLONING AND SEQUENCE-ANALYSIS OF A CDNA-ENCODING A BRAZIL NUT PROTEIN EXCEPTIONALLY RICH IN METHIONINE [J].

ALTENBACH, SB ;

PEARSON, KW ;

LEUNG, FW ;

SUN, SSM .

PLANT MOLECULAR BIOLOGY, 1987, 8 (03) :239-250

[2] THE AMINO-ACID-SEQUENCE OF THE 2S SULFUR-RICH PROTEINS FROM SEEDS OF BRAZIL NUT (BERTHOLLETIA-EXCELSA HBK) [J].

AMPE, C ;

VANDAMME, J ;

DECASTRO, LAB ;

SAMPAIO, MJAM ;

VANMONTAGU, M ;

VANDEKERCKHOVE, J .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 159 (03) :597-604

[3]

CROUCH M L, 1983, Journal of Molecular and Applied Genetics, V2, P273

[4] EVIDENCE FOR A PRECURSOR MOLECULE OF BRAZIL NUT 2-S SEED PROTEINS FROM BIOSYNTHESIS AND CDNA ANALYSIS [J].

DECASTRO, LAB ;

LACERDA, Z ;

ARAMAYO, RA ;

SAMPAIO, MJAM ;

GANDER, ES .

MOLECULAR & GENERAL GENETICS, 1987, 206 (02) :338-343

[5] TISSUE SULFHYDRYL GROUPS [J].

ELLMAN, GL .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1959, 82 (01) :70-77

[6]

ERICSON ML, 1986, J BIOL CHEM, V261, P4576

[7]

GLAZER AN, 1975, CHEM MODIFICATION PR, P101

[8]

HIGGINBOTHAM JD, 1983, DEV SWEETENERS, V2, P115

[9] THE RICINUS-COMMUNIS 2S ALBUMIN PRECURSOR - A SINGLE PREPROPROTEIN MAY BE PROCESSED INTO 2 DIFFERENT HETERODIMERIC STORAGE PROTEINS [J].

IRWIN, SD ;

KEEN, JN ;

FINDLAY, JBC ;

LORD, JM .

MOLECULAR & GENERAL GENETICS, 1990, 222 (2-3) :400-408

[10]

JOSEFSSON LG, 1987, J BIOL CHEM, V262, P12196

← 1 2 →