DETECTION AND REMOVAL OF ESCHERICHIA-COLI USING FLUORESCEIN ISOTHIOCYANATE CONJUGATED MONOCLONAL-ANTIBODY IMMOBILIZED ON BACTERIAL MAGNETIC PARTICLES

被引：86

作者：

NAKAMURA, N ^{[1
]}

BURGESS, JG ^{[1
]}

YAGIUDA, K ^{[1
]}

KUDO, S ^{[1
]}

SAKAGUCHI, T ^{[1
]}

MATSUNAGA, T ^{[1
]}

机构：

[1] TOKYO UNIV AGR & TECHNOL, DEPT BIOTECHNOL, KOGANEI, TOKYO 184, JAPAN

来源：

ANALYTICAL CHEMISTRY | 1993年 / 65卷 / 15期

关键词：

D O I：

10.1021/ac00063a018

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel fluoroimmunoassay method using bacterial magnetic particles for the highly sensitive detection of bacteria has been developed. Fluorescein isothiocyanate (FITC) conjugated monoclonal anti-Escherichia coli antibody was immobilized onto bacterial magnetic particles (BMPs) using a heterobifunctional reagent, N-succinimdyl 3-(2-pyridyldithio)propionate(SPDP). E. coli cells were reacted with FITC-antibody-BMP conjugates for 15 min in an inhomogeneous magnetic field which enhanced aggregation. The cell/BMP complexes sedimented, causing relative fluorescence intensity of the solution to decrease with increasing microbial cell concentration. A linear relationship was obtained between the relative fluorescence intensity and cell concentration in the range of 10(2)-10(6) cells/mL. Selectivity of this detection system was satisfactory. Monoclonal antibody immobilized on BMPs was also applied to the specific removal of E. coli from the bacterial suspension.

引用

页码：2036 / 2039

页数：4

共 17 条

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