HAIRY ROOT TRANSFORMATION IN ALFALFA (MEDICAGO-SATIVA L)

被引：78

作者：

SPANO, L ^{[1
]}

MARIOTTI, D ^{[1
]}

PEZZOTTI, M ^{[1
]}

DAMIANI, F ^{[1
]}

ARCIONI, S ^{[1
]}

机构：

[1] FAC AGR PERUGIA, CNR, CTR STUDIO MIGLIORAMENTO GENET PIANTE FORAGGERE, I-06100 PERUGIA, ITALY

来源：

THEORETICAL AND APPLIED GENETICS | 1987年 / 73卷 / 04期

关键词：

D O I：

10.1007/BF00289189

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The widely cultivated forage legume alfalfa (Medicago sativa L.) was transformed with the agropine type Agrobacterium rhizogenes NCPPB 1855. Sterile root and callus cultures were derived from tumorous hairy roots which were easily obtained independent of the plant variety or genotype. Plant regeneration, via somatic embryogenesis, was achieved only when a selected alfalfa line, characterized by high regenerative capability, was utilized. Genetic transformation was confirmed by the presence of agropine and T-DNA. Phenotypic alterations, mainly affecting the root system, were observed in transformed plants. The possibility that T-DNA-induced variations could be useful in the improvement of M. sativa is discussed.

引用

页码：523 / 530

页数：8

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