INDUCTION OF IMMEDIATE-EARLY GENES BY CYCLIC-AMP IN PRIMARY CULTURES OF NEURONS FROM RAT CEREBRAL-CORTEX

被引：44

作者：

VACCARINO, FM ^{[1
]}

HAYWARD, MD ^{[1
]}

LE, HN ^{[1
]}

HARTIGAN, DJ ^{[1
]}

DUMAN, RS ^{[1
]}

NESTLER, EJ ^{[1
]}

机构：

[1] YALE UNIV, SCH MED, DEPT PSYCHIAT, NEW HAVEN, CT 06510 USA

来源：

MOLECULAR BRAIN RESEARCH | 1993年 / 19卷 / 1-2期

关键词：

C-FOS; C-JUN; NGFI-A; CEREBRAL CORTEX; PROTOONCOGENE; VASOACTIVE INTESTINAL PEPTIDE;

D O I：

10.1016/0169-328X(93)90151-E

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In this paper, we tested whether physiological activators of the cAMP second messenger pathway in primary cultures of neurons from rat cerebral cortex directly induce c-fos and other immediate early gene (IEG) transcription factors. We have found that brief (30 s to 2 min) stimulation of neurons with vasoactive intestinal peptide (VIP) and SKF-38393, a D1-dopaminergic receptor agonist, potently increased mRNA levels for the IEGs c-fos, jun-B, and NGFI-A, with weaker increases for c-jun. This action was mimicked by forskolin and dibutyryl cAMP. IEG induction by VIP and dibutyryl cAMP was not blocked by excitatory amino acid receptor antagonists or by blockers of dihydropyridine-sensitive calcium channels. Moreover, calcium-free medium did not modify IEG induction by dibutyryl cAMP, suggesting that cAMP can directly regulate IEG expression in differentiated neurons independently of calcium.

引用

页码：76 / 82

页数：7

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