MUTATIONAL ANALYSIS OF SCHIZOSACCHAROMYCES-POMBE U4 SNRNA BY PLASMID EXCHANGE

被引：4

作者：

DANDEKAR, T

TOLLERVEY, D

机构：

[1] Embl, Heidelberg, D-6900

来源：

YEAST | 1992年 / 8卷 / 08期

关键词：

SCHIZOSACCHAROMYCES-POMBE; SNRNA; SNRNP; U4;

D O I：

10.1002/yea.320080808

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a system for testing mutations by plasmid exchange in the fission yeast Schizosaccharomyces pombe. This system has been used to test the requirement for different regions of the small nuclear RNA U4 in S. pombe. Surprisingly, five of seven deletion and substitution mutations tested in different regions of U4 prevent the accumulation of the mutant RNA. Substitution of the U4 sequence in stem 1 of the U4/U6 interaction domain allows accumulation of the mutant U4, but does not support viability. Two sequences with homology to the Sm binding site are found in the 3' region of S.pombe U4; substitution of the 3' sequence of the two does not interfere with accumulation or function of U4, indicating that the 5' sequence is the functional Sm-binding site.

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页码：647 / 653

页数：7

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