THE MONOCLONAL-ANTIBODY MAC387 DETECTS AN EPITOPE ON THE CALCIUM-BINDING PROTEIN MRP14

被引:50
作者
GOEBELER, M
ROTH, J
TEIGELKAMP, S
SORG, C
机构
[1] Inst. of Experimental Dermatology, University of Munster, 48149 Munster
关键词
MRP8; 1-COMPLEX; S100-PROTEIN; 27EIO; MONOCYTE/MACROPHAGE SYSTEM;
D O I
10.1002/jlb.55.2.259
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The present study was initiated to identify the antigen recognized by the monoclonal antibody (MAb) MAC387 which is widely used for phenotypical characterization of myelomonocytic cells in situ. MAC387 has been described to show a similar reaction pattern as antisera to a complex formed by the calcium-binding proteins MRP8 and MRP14. However, the exact nature of the molecule recognized by MAC387 has been controversial. Using Western blot analysis, MAC387 was found to detect a single protein band of 14 kDa in lysates of human monocytes and granulocytes. Transfection of embryonic lung fibroblasts L132 with either MRP8 or MRP14 cDNA revealed that MAC387 reacts with MRP14 but not MRP8. This finding was confirmed by dot blot analysis of recombinant MRP8 and MRP14. Our data thus provide unequivocal evidence that MAC387 is a monoclonal antibody directed against the calcium-binding protein MRP14.
引用
收藏
页码:259 / 261
页数:3
相关论文
共 17 条
[1]   THE CALCIUM-BINDING PROTEINS MRP8 AND MRP14 FORM A MEMBRANE-ASSOCIATED HETERODIMER IN A SUBSET OF MONOCYTES MACROPHAGES PRESENT IN ACUTE BUT ABSENT IN CHRONIC INFLAMMATORY LESIONS [J].
BHARDWAJ, RS ;
ZOTZ, C ;
ZWADLOKLARWASSER, G ;
ROTH, J ;
GOEBELER, M ;
MAHNKE, K ;
FALK, M ;
MEINARDUSHAGER, G ;
SORG, C .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1992, 22 (07) :1891-1897
[2]   MAC-387 ANTIBODY AND DETECTION OF FORMALIN RESISTANT MYELOMONOCYTIC L1 ANTIGEN [J].
BRANDTZAEG, P ;
JONES, DB ;
FLAVELL, DJ ;
FAGERHOL, MK .
JOURNAL OF CLINICAL PATHOLOGY, 1988, 41 (09) :963-970
[3]   THE LEUKOCYTE PROTEIN-L1 (CALPROTECTIN) - USEFULNESS AS AN IMMUNOHISTOCHEMICAL MARKER ANTIGEN AND PUTATIVE BIOLOGICAL FUNCTION [J].
BRANDTZAEG, P ;
DALE, I ;
GABRIELSEN, TO .
HISTOPATHOLOGY, 1992, 21 (02) :191-196
[4]  
DELABIE J, 1990, CLIN EXP IMMUNOL, V81, P123
[5]  
EDGEWORTH J, 1991, J BIOL CHEM, V266, P7706
[6]   IDENTIFICATION OF TISSUE HISTIOCYTES ON PARAFFIN SECTIONS BY A NEW MONOCLONAL-ANTIBODY [J].
FLAVELL, DJ ;
JONES, DB ;
WRIGHT, DH .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1987, 35 (11) :1217-1226
[7]   EXPRESSION AND COMPLEX ASSEMBLY OF CALCIUM-BINDING PROTEIN-MRP8 AND PROTEIN-MRP14 DURING DIFFERENTIATION OF MURINE MYELOMONOCYTIC CELLS [J].
GOEBELER, M ;
ROTH, J ;
HENSELEIT, U ;
SUNDERKOTTER, C ;
SORG, C .
JOURNAL OF LEUKOCYTE BIOLOGY, 1993, 53 (01) :11-18
[8]   MRP-8 AND MRP-14, 2 ABUNDANT CA-2+-BINDING PROTEINS OF NEUTROPHILS AND MONOCYTES [J].
HESSIAN, PA ;
EDGEWORTH, J ;
HOGG, N .
JOURNAL OF LEUKOCYTE BIOLOGY, 1993, 53 (02) :197-204
[9]   MORPHOLOGICAL EVIDENCE FOR CALCIUM-DEPENDENT ASSOCIATION OF CALGRANULIN WITH THE EPIDERMAL CYTOSKELETON IN INFLAMMATORY DERMATOSES [J].
KELLY, SE ;
HUNTER, JAA ;
JONES, DB ;
CLARK, BR ;
FLEMING, S .
BRITISH JOURNAL OF DERMATOLOGY, 1991, 124 (05) :403-409
[10]  
KUWAYAMA A, 1993, BLOOD, V81, P3116