THE KINETICS OF THE ACID-INDUCED CONFORMATIONAL CHANGE OF INFLUENZA-VIRUS HEMAGGLUTININ CAN BE FOLLOWED USING 1,1'-BIS(4-ANILINO-5-NAPHTHALENESULPHONIC ACID)

被引:11
作者
BETHELL, RC
GRAY, NM
PENN, CR
机构
[1] Glaxo Grp Res Ltd, Dept Virol, Greenford, Middx England
关键词
D O I
10.1006/bbrc.1995.1049
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
1,1'-Bis(4-anilino-5-naphthalenesulphonic acid) (bis-ANS) has been shown by fluorescence spectroscopy to bind to bromelain-cleaved influenza haemagglutinin (BHA). The fluorescence intensity of 1.2 mu M bis-ANS in the presence of BHA in its low-pH conformation is twenty-fold higher than in the presence of BHA in its neutral-pH conformation. The use of this probe provides a sensitive method for investigating the kinetics of the irreversible conformational change of BHA induced by low pH. At pH5.0 the reaction is described by a rapid burst followed by a double exponential increase in the fluorescence of bis-ANS, with rate constants of 5.2+/-0.9x10(-3)sec(-1) and 6.7+/-1.9x10(-4)sec(-1). This reaction is sensitive to the presence of tert-butylhydroquinone, an inhibitor of the conformational transition of BHA. The dependence of the reaction rate on pH indicates that the acid-induced conformational change is dependent upon the multiple protonation of the neutral-pH conformation of BHA. (C) 1995 Academic Press, Inc.
引用
收藏
页码:355 / 361
页数:7
相关论文
共 14 条
  • [1] INHIBITION OF THE FUSION-INDUCING CONFORMATIONAL CHANGE OF INFLUENZA HEMAGGLUTININ BY BENZOQUINONES AND HYDROQUINONES
    BODIAN, DL
    YAMASAKI, RB
    BUSWELL, RL
    STEARNS, JF
    WHITE, JM
    KUNTZ, ID
    [J]. BIOCHEMISTRY, 1993, 32 (12) : 2967 - 2978
  • [2] CRYSTALLINE ANTIGEN FROM INFLUENZA-VIRUS ENVELOPE
    BRAND, CM
    SKEHEL, JJ
    [J]. NATURE-NEW BIOLOGY, 1972, 238 (83): : 145 - &
  • [3] STRUCTURE OF INFLUENZA HEMAGGLUTININ AT THE PH OF MEMBRANE-FUSION
    BULLOUGH, PA
    HUGHSON, FM
    SKEHEL, JJ
    WILEY, DC
    [J]. NATURE, 1994, 371 (6492) : 37 - 43
  • [4] A SPRING-LOADED MECHANISM FOR THE CONFORMATIONAL CHANGE OF INFLUENZA HEMAGGLUTININ
    CARR, CM
    KIM, PS
    [J]. CELL, 1993, 73 (04) : 823 - 832
  • [5] DOMS RW, 1985, J BIOL CHEM, V260, P2973
  • [6] KORTE T, 1994, EUR BIOPHYS J BIOPHY, V23, P105
  • [7] LI S, 1994, BIOCHEMISTRY-US, V33, P7536
  • [8] FLUOROMETRIC ASSAY OF NEURAMINIDASE WITH A SODIUM (4-METHYLUMBELLIFERYL-ALPHA-D-N-ACETYLNEURAMINATE) SUBSTRATE
    POTIER, M
    MAMELI, L
    BELISLE, M
    DALLAIRE, L
    MELANCON, SB
    [J]. ANALYTICAL BIOCHEMISTRY, 1979, 94 (02) : 287 - 296
  • [9] HEMAGGLUTININS FROM 2 INFLUENZA-VIRUS VARIANTS BIND TO SIALIC-ACID DERIVATIVES WITH MILLIMOLAR DISSOCIATION-CONSTANTS - A 500-MHZ PROTON NUCLEAR MAGNETIC-RESONANCE STUDY
    SAUTER, NK
    BEDNARSKI, MD
    WURZBURG, BA
    HANSON, JE
    WHITESIDES, GM
    SKEHEL, JJ
    WILEY, DC
    [J]. BIOCHEMISTRY, 1989, 28 (21) : 8388 - 8396
  • [10] CHANGES IN THE CONFORMATION OF INFLUENZA-VIRUS HEMAGGLUTININ AT THE PH OPTIMUM OF VIRUS-MEDIATED MEMBRANE-FUSION
    SKEHEL, JJ
    BAYLEY, PM
    BROWN, EB
    MARTIN, SR
    WATERFIELD, MD
    WHITE, JM
    WILSON, IA
    WILEY, DC
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (04): : 968 - 972