ALL YOU WANTED TO KNOW ABOUT SELEX

被引：221

作者：

KLUG, SJ ^{[1
]}

FAMULOK, M ^{[1
]}

机构：

[1] UNIV MUNICH,INST MOLEK BIOCHEM,D-81375 MUNICH,GERMANY

来源：

MOLECULAR BIOLOGY REPORTS | 1994年 / 20卷 / 02期

关键词：

IN VITRO SELECTION; SELEX; COMBINATORIAL NUCLEIC ACID LIBRARIES; APTAMERS; RIBOZYMES;

D O I：

10.1007/BF00996358

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In vitro selection, or SELEX, is a technique that allows the simultaneous screening of highly diverse pools of different RNA or DNA (dsDNA or ssDNA) molecules for a particular feature. Different examples from a great variety of applications of in vitro selection experiments are described and a detailed overview of the method and its variations will be given. Some especially conclusive in vitro selection experiments are discussed in detail to illustrate the potential power and diversity of this method. Potential restrictions of the methods and possible ways to overcome them are pointed out.

引用

页码：97 / 107

页数：11

共 59 条

[1] HIV-1 REV REGULATION INVOLVES RECOGNITION OF NON-WATSON-CRICK BASE-PAIRS IN VIRAL-RNA [J].