MULTIPLE COMMON FRAGILE SITES ARE EXPRESSED IN THE GENOME OF THE LABORATORY RAT

被引：50

作者：

ROBINSON, TJ ^{[1
]}

ELDER, FFB ^{[1
]}

机构：

[1] BAYLOR COLL MED, INST MOLEC GENET, HOUSTON, TX 77030 USA

来源：

CHROMOSOMA | 1987年 / 96卷 / 01期

关键词：

D O I：

10.1007/BF00285882

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Splenic lymphocytes from Sprague Dawley and Fischer 344 rats were exposed to two chemicals known to induce common fragile site expression in man: fluorodeoxyuridine (in conjunction with the enhancing effects of caffeine) and aphidicolin. Of 39 sites that were significantly damaged in excess, 12 meet the criteria for fragility proposed in this investigation. Rat fragile sites appear to differ from those in man in that no common hierarchical frequency of expression is evident from the two methods of induction. In addition, a comparison of published cancer-specific chromosome breakpoints from a variety of rat tumors reveals little or no apparent concordance with the identified fragile sites. The rat is an animal model in which multiple common fragile sites can be induced and, as such, will be valuable for testing hypotheses concerning the biological basis of chromosomal fragility.

引用

页码：45 / 49

页数：5

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