DETERMINATION OF LEVELS OF CYCLIC-AMP IN THE MYENTERIC PLEXUS OF GUINEA-PIG SMALL-INTESTINE

被引:35
作者
XIA, Y
BAIDAN, LV
FERTEL, RH
WOOD, JD
机构
[1] OHIO STATE UNIV,COLL MED,DEPT PHYSIOL,4196 GRAVES HALL,333 W 10TH AVE,COLUMBUS,OH 43210
[2] OHIO STATE UNIV,COLL MED,DEPT PHARMACOL,COLUMBUS,OH 43210
来源
EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION | 1991年 / 206卷 / 03期
关键词
CAMP; ENTERIC NERVOUS SYSTEM; MYENTERIC NEURONS; INTESTINE; SYNAPTIC TRANSMISSION; FORSKOLIN;
D O I
10.1016/S0922-4106(05)80023-9
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Enzymatically dissociated ganglia from the myenteric plexus of the guinea-pig small intestine were used to investigate changes in levels of cyclic 3',5'-adenosine monophosphate (cAMP) in response to stimulation of adenylate cyclase by forskolin and inhibition of phosphodiesterase by 3-isobutyl-1-methylxanthine (IBMX). A linear relation with a positive correlation coefficient greater than 0.98 was found between: (1) amount of cAMP and number of ganglia; (2) amount of protein and number of ganglia; (3) amount of DNA and amount of protein; (4) amount of DNA and number of ganglia. Basal levels of cAMP were 2.25 +/- 0.21 fmol per ganglion for 900 ganglia. Forskolin stimulated a dose-dependent increase in cAMP over a concentration range of 0.05 to 50-mu-M, with a level of 18.6 +/- 4.9 fmol/ganglion at 50-mu-M forskolin. The inactive forskolin analog 1,9-dideoxyforskolin did not elevate cAMP. Addition of IBMX to the incubation medium stimulated a dose-dependent increase in cAMP over a concentration range of 0.1-1000-mu-M, with a level of 17.58 +/- 3.38 fmol/ganglion at 1000-mu-M IBMX. Application of 1 mM IBMX strongly potentiated the stimulating action of forskolin on cAMP levels. Our results derived from direct determination of cAMP changes in small intestinal myenteric ganglia are consistent with existing electrophysiological evidence for second messenger function of cAMP in slow synaptic modulation of excitability in AH/Type 2 neurons of the enteric nervous system.
引用
收藏
页码:231 / 236
页数:6
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