MOLECULAR-BASIS FOR FIBRINOGEN DUSART (A-ALPHA 554 ARG -] CYS) AND ITS ASSOCIATION WITH ABNORMAL FIBRIN POLYMERIZATION AND THROMBOPHILIA

被引:106
作者
KOOPMAN, J
HAVERKATE, F
GRIMBERGEN, J
LORD, ST
MOSESSON, MW
DIORIO, JP
SIEBENLIST, KS
LEGRAND, C
SORIA, J
SORIA, C
CAEN, JP
机构
[1] TNO,IVVO,GAUBIUS LAB,POB 430,2300 AK LEIDEN,NETHERLANDS
[2] LEIDEN UNIV HOSP,DEPT HEMATOL,2333 AA LEIDEN,NETHERLANDS
[3] UNIV N CAROLINA,DEPT PATHOL,CHAPEL HILL,NC 27514
[4] UNIV WISCONSIN,SCH MED,SINAI SAMARITAN MED CTR,MILWAUKEE,WI 53233
[5] HOP LARIBOISIERE,INSERM,U150,F-75475 PARIS 10,FRANCE
[6] HOP LARIBOISIERE,INST VAISSEAUX & SANG,F-75475 PARIS 10,FRANCE
关键词
A-ALPHA CHAIN; ALBUMIN; DISULFIDE-LINKED; DYSFIBROGENEMIA; GENE ANALYSIS;
D O I
10.1172/JCI116371
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
The molecular defect in the abnormal fibrinogen Dusart (Paris V) that is associated with thrombophilia was determined by sequence analysis of genomic DNA that had been amplified using the polymerase chain reaction. The propositus was heterozygous for a single base change (C --> T) in the Aalpha-chain gene, resulting in the amino acid substitution Aalpha 554 Arg --> Cys. Restriction analysis of the amplified DNA derived from the family members showed that his father and his two sons were also heterozygous. Electron microscopic studies on fibrin formed from purified fibrinogen Dusart demonstrated fibers that were much thinner than in normal fibrin. In contrast to the previously observed defective binding of plasminogen, the binding of thrombospondin to immobilized fibrinogen Dusart was similar to that of normal fibrinogen. Immunoblot analysis of plasma fibrinogen demonstrated that a substantial part of the fibrinogen Dusart molecules were disulfide-linked to albumin. The plasma of the affected family members also contained fibrinogen-albumin complexes. Furthermore, small amounts of high molecular weight complexes containing fibrinogen were detected in all the heterozygous individuals. These data indicate that the molecular abnormality in fibrinogen Dusart (Aalpha 554 Arg --> Cys) results in defective lateral association of the fibrin fibers and disulfide-linked complex formation with albumin, and is associated with a family history of recurrent thrombosis in the affected individuals.
引用
收藏
页码:1637 / 1643
页数:7
相关论文
共 44 条
  • [1] ABILGAARD U, 1975, THROMB DIATH HAEMO, V6, P287
  • [2] BACONBAGULEY T, 1987, J BIOL CHEM, V262, P1927
  • [3] BLOMBACK B, 1956, ARK KEMI, V10, P415
  • [4] FIBRIN POLYMERIZATION SITES IN FIBRINOGEN AND FIBRIN FRAGMENTS
    BUDZYNSKI, AZ
    OLEXA, SA
    PANDYA, BV
    [J]. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1983, 408 (JUN) : 301 - 314
  • [5] CAEN J, 1975, HEMOSTASE METHODES E
  • [6] MASS-LENGTH RATIO OF FIBRIN FIBERS FROM GEL-PERMEATION AND LIGHT-SCATTERING
    CARR, ME
    SHEN, LL
    HERMANS, J
    [J]. BIOPOLYMERS, 1977, 16 (01) : 1 - 15
  • [7] CHARACTERIZATION OF COMPLEMENTARY DEOXYRIBONUCLEIC-ACID AND GENOMIC DEOXYRIBONUCLEIC-ACID FOR THE BETA-CHAIN OF HUMAN-FIBRINOGEN
    CHUNG, DW
    QUE, BG
    RIXON, MW
    MACE, M
    DAVIE, EW
    [J]. BIOCHEMISTRY, 1983, 22 (13) : 3244 - 3250
  • [8] CLAUSS A., 1957, ACTA HAEMATOL, V17, P237
  • [9] DUBERNARD V, 1991, J LAB CLIN MED, V118, P446
  • [10] Ebert RF, 1991, INDEX VARIANT HUMAN