NITROGEN TRANSFER IN ESCHERICHIA-COLI GLUCOSAMINE-6P SYNTHASE - INVESTIGATIONS USING SUBSTRATE AND BISUBSTRATE ANALOGS

被引：35

作者：

BADETDENISOT, MA ^{[1
]}

LERICHE, C ^{[1
]}

MASSIERE, F ^{[1
]}

BADET, B ^{[1
]}

机构：

[1] CNRS,INST CHIM SUBST NAT,F-91198 GIF SUR YVETTE,FRANCE

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1995年 / 5卷 / 08期

关键词：

D O I：

10.1016/0960-894X(95)00119-E

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The compound 2-amino-2-deoxyglucitol-6P competitively inhibits E. coli glucosamine-6P synthase with respect to fructose-6P whereas glutamate gamma-semialdehyde is a competitive inhibitor with respect to glutamine. These compounds, which exhibit good inhibitory properties (K-m/K-i= 16 and 1000 respectively), were used in the synthesis of multisubstrate analogs.

引用

页码：815 / 820

页数：6

共 27 条

[1] GLUCOSAMINE SYNTHETASE FROM ESCHERICHIA-COLI - PURIFICATION, PROPERTIES, AND GLUTAMINE-UTILIZING SITE LOCATION [J].

BADET, B ;

VERMOOTE, P ;

HAUMONT, PY ;

LEDERER, F ;

LEGOFFIC, F .

BIOCHEMISTRY, 1987, 26 (07) :1940-1948

[2] GLUCOSAMINE SYNTHETASE FROM ESCHERICHIA-COLI - KINETIC MECHANISM AND INHIBITION BY N3-FUMAROYL-L-2,3-DIAMINOPROPIONIC DERIVATIVES [J].

BADET, B ;

VERMOOTE, P ;

LEGOFFIC, F .

BIOCHEMISTRY, 1988, 27 (07) :2282-2287

[3]

BADETDENISOT MA, 1993, B SOC CHIM FR, V130, P249

[4]

BODANSZKY M, 1981, SYNTHESIS-STUTTGART, P333

[5]

BODANZSKY M, 1984, PRACTICE PEPTIDE SYN

[6] MOLECULAR-CLONING AND OVEREXPRESSION OF THE GLUCOSAMINE SYNTHETASE GENE FROM ESCHERICHIA-COLI [J].

DUTKAMALEN, S ;

MAZODIER, P ;

BADET, B .

BIOCHIMIE, 1988, 70 (02) :287-290

[7]

GOLINELLIPIMPAN.B, 1991, EUR J BIOCHEM, V201, P175, DOI 10.1111/J.1432-1033.1991.TB16271.X

[8]

GOLINELLIPIMPAN.B, 1989, J AM CHEM SOC, V111, P3029

[9] GENETIC AND BIOCHEMICAL-STUDIES DEMONSTRATING A 2ND GENE CODING FOR ASPARAGINE SYNTHETASE IN ESCHERICHIA-COLI [J].

HUMBERT, R ;

SIMONI, RD .

JOURNAL OF BACTERIOLOGY, 1980, 142 (01) :212-220

[10] DIRECT EVIDENCE FOR AN ACYLATED THIOL AS IN INTERMEDIATE IN PAPAIN- AND FICIN-CATALYSED HYDROLYSES [J].

LOWE, G ;

WILLIAMS, A .

BIOCHEMICAL JOURNAL, 1965, 96 (01) :189-&

← 1 2 3 →