THE EFFECT OF DITHIOTREITOL ON THYROID PEROXIDASE AND MICROSOMAL ANTIGEN EPITOPES RECOGNIZED BY AUTO AND MONOCLONAL-ANTIBODIES

被引：12

作者：

GARDAS, A

DOMEK, H

CZARNOCKA, B

机构：

[1] Biochemistry Department, Medical Center of Postgraduate Education, 01-813, Warsaw

来源：

AUTOIMMUNITY | 1990年 / 7卷 / 2-3期

关键词：

Dithiotreitol sensitivity; Thyroid peroxidase epitopes;

D O I：

10.3109/08916939008993387

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The effect of disulphide bridges reduction of the microsomal antigen (Mic-Ag) and thyroid peroxidase (TPO) by dithiotreitol (DTT) has been investigated. The reaction of all 67 tested sera from untreated hyperthyroid Graves' and from 22 Hashimoto's patients with high microsomal antibodies (aAb) titer was diminished by 90-95% by DTT, at pH 9.6. The remaining S-10%of the activity was not destroyed by DTT. The residual Mic-Ag after DTT reduction was able to inhibit the binding of all 45 Graves' and 22 Hashimoto's tested aAb's to the native microsomal antigen by 100% at high concentration. Reaction of affinity purified TPO with two monoclonal antibodies (mAb) were diminished by 80% to 95% by DTT pretreatment, while the reaction of one mAb with TPO was only slightly affected. The reaction of TPO and Mic-Ag with rabbit polyclonal anti-TPO serum (rabbit aTPO) was diminished by 60% by DTT pretreatment. The immunological reactivity of TPO with aAb's was diminished by 65% after DTT pretreatment. The microsomal antigen-aAb's complex was not destroyed by DTT. Results presented in this paper suggest conformational epitope structure of the Mic-Ag recognized by aAb's in patients with thyroid autoimmune disease (AITD). © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：149 / 156

页数：8

共 14 条

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