LINKAGE OF RIBOSOMAL-RNA GENES IN LEPTOSPIRA

被引：22

作者：

FUKUNAGA, M ^{[1
]}

MASUZAWA, T ^{[1
]}

OKUZAKO, N ^{[1
]}

MIFUCHI, I ^{[1
]}

YANAGIHARA, Y ^{[1
]}

机构：

[1] UNIV SHIZUOKA,SCH PHARMACEUT SCI,DEPT MICROBIOL,YADA,SHIZUOKA 422,JAPAN

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1990年 / 34卷 / 07期

关键词：

D O I：

10.1111/j.1348-0421.1990.tb01033.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We determined the linkage of 16S, 23S, and 5S rRNA genes in several strains of Leptospira and Leptonema by DNA-DNA hybridization. Almost all the hybridizations in all leptospires used in these experiments gave two radioactive bands and the results strongly suggest that the number of the 16S and the 23S rRNA genes in those strains is two, respectively. In contrast with the larger rRNAs, the number of 5S rRNA gene was different. In the strains of leptospires, L. biflexa, which were non-parasitic, there are two genes for 5S rRNA, whereas only one gene for 5S rRNA is carried in L. interrogans, which were originally isolated as parasitic. Southern hybridization experiments suggest that those rRNA genes are interspersed on the leptospiral chromosome. © 1990, Center For Academic Publications Japan. All rights reserved.

引用

页码：565 / 573

页数：9

共 27 条

[1] RIBOSOMAL-RNA GENES IN MYCOPLASMA [J].

AMIKAM, D ;

RAZIN, S ;

GLASER, G .

NUCLEIC ACIDS RESEARCH, 1982, 10 (14) :4215-4222

[2] PHYSICAL MAP OF THE 7 RIBOSOMAL-RNA GENES OF ESCHERICHIA-COLI [J].

BOROS, I ;

KISS, A ;

VENETIANER, P .

NUCLEIC ACIDS RESEARCH, 1979, 6 (05) :1817-1830

[3]

BOTT KF, 1984, GENETICS BIOTECHNOLO, P19

[4] NOVEL ARRANGEMENT OF RIBOSOMAL-RNA GENES IN MYCOPLASMA-GALLISEPTICUM - SEPARATION OF THE 16S-GENE OF ONE SET FROM THE 23S-GENE AND 5S-GENE [J].

CHEN, X ;

FINCH, LR .

JOURNAL OF BACTERIOLOGY, 1989, 171 (05) :2876-2878

[5] UNIQUE ORGANIZATION OF LEPTOSPIRA-INTERROGANS RIBOSOMAL-RNA GENES [J].

FUKUNAGA, M ;

MIFUCHI, I .

JOURNAL OF BACTERIOLOGY, 1989, 171 (11) :5763-5767

[6] NUCLEOTIDE-SEQUENCE OF A 23S RIBOSOMAL-RNA GENE FROM LEPTOSPIRA-INTERROGANS SEROVAR CANICOLA STRAIN MOULTON [J].

FUKUNAGA, M ;

HORIE, I ;

MIFUCHI, I .

NUCLEIC ACIDS RESEARCH, 1989, 17 (05) :2123-2123

[7] THE NUMBER OF LARGE RIBOSOMAL-RNA GENES IN LEPTOSPIRA-INTERROGANS AND LEPTOSPIRA-BIFLEXA [J].

FUKUNAGA, M ;

MIFUCHI, I .

MICROBIOLOGY AND IMMUNOLOGY, 1989, 33 (06) :459-466

[8] ISOLATION AND CHARACTERIZATION OF THE 5S RIBOSOMAL-RNA GENE OF LEPTOSPIRA-INTERROGANS [J].

FUKUNAGA, M ;

HORIE, I ;

MIFUCHI, I .

JOURNAL OF BACTERIOLOGY, 1990, 172 (06) :3264-3268

[9]

FUKUNAGA M, 1988, NUCLEIC ACIDS RES, V18, P366

[10] SPIROCHETES [J].

JOHNSON, RC .

ANNUAL REVIEW OF MICROBIOLOGY, 1977, 31 :89-106

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