COMPARATIVE-STUDY OF A DNA HYBRIDIZATION METHOD AND 2 ISOLATION PROCEDURES FOR DETECTION OF YERSINIA-ENTEROCOLITICA O-3 IN NATURALLY CONTAMINATED PORK PRODUCTS

被引:43
作者
NESBAKKEN, T
KAPPERUD, G
DOMMARSNES, K
SKURNIK, M
HORNES, E
机构
[1] NATL INST PUBL HLTH,DEPT BACTERIOL,N-0462 OSLO 4,NORWAY
[2] TURKU UNIV,DEPT MED MICROBIOL,SF-20520 TURKU,FINLAND
[3] NORWEGIAN COLL VET MED,DEPT FOOD HYG,N-0033 OSLO 1,NORWAY
关键词
D O I
10.1128/AEM.57.2.389-394.1991
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We compared a DNA-DNA hybridization assay, using a synthetically produced oligonucleotide probe, and two conventional isolation procedures (methods A and B) with regard to their relative efficiency in detecting Yersinia enterocolitica O:3 in naturally contaminated pork products. Method A was as described by Wauters et al. (Appl. Environ. Microbiol. 54:851-854, 1988). Method B has been recommended by the Nordic Committee on Food Analysis (method no. 117, 1987). The genetic probe was used in a colony hybridization assay to detect virulent yersiniae at each of the isolation steps which composed methods A and B. A total of 50 samples of raw pork products obtained from 13 meat-processing factories in Norway were examined. Y. enterocolitica serogroup O:3, biovar 4, was isolated from altogether 9 (18.0%) of the samples by using the two isolation procedures. In contrast, colony hybridization using the genetic probe indicated that 30(60.0%) of the samples contained virulent yersiniae. All samples which were positive on cultivation were also positive by hybridization. The results indicate that the occurrence of pathogenic Y. enterocolitica in Norwegian pork products is substantially higher than previously demonstrated and, therefore, reinforce our suggestion that pork products represent an important potential source of human infection in Norway. The results also indicate that the use of conventional isolation procedures may lead to considerable underestimation of pathogenic Y. enterocolitica in pork products.
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页码:389 / 394
页数:6
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