RHODAMINE-LABELED PHALLOIDIN STAINS COMPONENTS IN THE CHROMOSOMAL SPINDLE FIBERS OF CRANE-FLY SPERMATOCYTES AND HAEMANTHUS ENDOSPERM CELLS

被引：34

作者：

CZABAN, BB

FORER, A

机构：

[1] Department of Biology, York University, Ont.

来源：

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE | 1992年 / 70卷 / 08期

关键词：

PHALLOIDIN; CHROMOSOMAL SPINDLE FIBERS; KINETOCHORES;

D O I：

10.1139/o92-102

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In crane-fly spermatocytes and Haemanthus endosperm, all metaphase and anaphase chromosomal spindle fibres were stained with rhodamine-labelled phalloidin. In crane-fly spermatocytes, each kinetochore was stained with rhodamine-labelled phalloidin at diakinesis of prophase and after colcemid caused metaphase spindles to depolymerize. Since phalloidin stains actin filaments, the distributions of rhodamine-labelled phalloidin-stained material in crane-fly spermatocytes and Haemanthus endosperm suggest that actin filaments might interact with microtubules to produce forces that move chromosomes during cell division, either directly or via an intermediate motor molecule.

引用

页码：664 / 676

页数：13

共 67 条

[1] MICROTUBULE-ASSOCIATED PROTEINS (MAPS) - A MONOCLONAL-ANTIBODY TO MAP 1 DECORATES MICROTUBULES INVITRO BUT STAINS STRESS FIBERS AND NOT MICROTUBULES INVIVO [J].