THERMODYNAMICS OF THE INTERACTION OF BERBERINE WITH DNA

被引：32

作者：

KUMAR, GS ^{[1
]}

DEBNATH, D ^{[1
]}

SEN, A ^{[1
]}

MAITI, M ^{[1
]}

机构：

[1] INDIAN INST CHEM BIOL,BIOPHYS CHEM LAB,4 RAJA S C MULLICK RD,CALCUTTA 700032,W BENGAL,INDIA

来源：

BIOCHEMICAL PHARMACOLOGY | 1993年 / 46卷 / 09期

关键词：

D O I：

10.1016/0006-2952(93)90337-V

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The interaction of berberine [7,8,13,13a-tetrahydro-9-10-dimethoxy-2,3-(methylene dioxy)-berberinium] with calf thymus DNA has been studied by spectrophotometry in buffers of various salt concentrations and temperatures. Binding parameters obtained are best fit by the neighbour exclusion model. The salt and temperature dependence of the binding constants arc used to estimate thermodynamic parameters involved in the complex formation of berberine with DNA. The binding process is exothermic over the entire range and the values of enthalpy and entropy change are strongly dependent on the salt concentration. The negative enthalpy and positive entropy changes compensate one another to produce a relatively small Gibbs' free energy change. Possible molecular contribution to the enthalpy and entropy changes is discussed.

引用

页码：1666 / 1667

页数：2

共 17 条

[1] THERMODYNAMIC STUDIES ON THE INTERACTIONS OF DI-SUBSTITUTED ANTHRAQUINONES WITH DNA [J].

BELL, A ;

BROWN, JR ;

NEIDLE, S .

BIOCHEMICAL PHARMACOLOGY, 1989, 38 (01) :216-217

[2]

BRESLAUER KJ, 1988, STRUCTURE EXPRESSION, V2, P273

[3] THERMODYNAMICS OF THE DAUNOMYCIN-DNA INTERACTION - IONIC-STRENGTH DEPENDENCE OF THE ENTHALPY AND ENTROPY [J].

CHAIRES, JB .

BIOPOLYMERS, 1985, 24 (02) :403-419

[4] THERMODYNAMICS OF THE INTERACTION OF ARISTOLOLACTAM-BETA-D-GLUCOSIDE WITH DNA - IONIC-STRENGTH DEPENDENCE OF ENTHALPY AND ENTROPY [J].

CHAKRABORTY, S ;

NANDI, R ;

MAITI, M .

BIOCHEMICAL PHARMACOLOGY, 1990, 39 (07) :1181-1186

[5] BIOCHEMICAL EFFECTS OF BERBERINE [J].

CREASEY, WA .

BIOCHEMICAL PHARMACOLOGY, 1979, 28 (07) :1081-1084

[6]

DEBNATH D, 1989, INDIAN J BIOCHEM BIO, V26, P201

[7] CIRCULAR-DICHROISM STUDIES OF THE STRUCTURE OF DNA COMPLEX WITH BERBERINE [J].

DEBNATH, D ;

KUMAR, GS ;

MAITI, M .

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 1991, 9 (01) :61-79

[8] ENTHALPY AND ENTROPY CHANGES FOR THE INTERCALATION OF SMALL MOLECULES TO DNA .2. ETHIDIUM AND PROPIDIUM FLUORIDE [J].

HOPKINS, HP ;

WILSON, WD .

BIOPOLYMERS, 1987, 26 (08) :1347-1355

[9]

KUMAR GS, 1992, ANTI-CANCER DRUG DES, V7, P305

[10]

MAITI M, 1981, INDIAN J BIOCHEM BIO, V18, P245

← 1 2 →