PROTEIN-SYNTHESIS USING S-ALKYL THIOESTER OF PARTIALLY PROTECTED PEPTIDE SEGMENTS - SYNTHESIS OF DNA-BINDING PROTEIN OF BACILLUS-STEAROTHERMOPHILUS

Using partially protected peptide thioesters as building blocks, we synthesized HU-type DNA-binding protein of Bacillus stearothermophilus. Four peptide segments, Boc-[Lys(Boc)3]-HBs(1-15)-SCH2CH2CONH2, iNoc-[Lys(Boc)18,19,23,38]-HBs(16-39)-SCH2CH2CONH2, iNoc-[Lys(Boc)41,59]-HBs(40-60)-SCH2CH2CONH2, [Lys(Boc)75,80,83,86,90]-HBs(61-90) were prepared using peptides obtained by a solid-phase method. A partially protected peptide thioester was condensed to a peptide with a free amino group by converting the thioester to the corresponding active ester in the presence of silver ions and N-hydroxysuccinimide. Finally, highly pure synthetic HBs(1-90) was obtained.