ELECTROPORATION AS A NEW TECHNIQUE FOR PRODUCING TRANSGENIC FISH

被引：95

作者：

INOUE, K ^{[1
]}

YAMASHITA, S ^{[1
]}

HATA, J ^{[1
]}

KABENO, S ^{[1
]}

ASADA, S ^{[1
]}

NAGAHISA, E ^{[1
]}

FUJITA, T ^{[1
]}

机构：

[1] NIPPON SUISAN KAISHA LTD,CENT RES LAB,HACHIOJI,TOKYO 192,JAPAN

来源：

CELL DIFFERENTIATION AND DEVELOPMENT | 1990年 / 29卷 / 02期

关键词：

Electroporation; Gene transfer; Growth hormone; Medaka; Microinjection; Transgenic fish;

D O I：

10.1016/0922-3371(90)90030-Z

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A recombinant plasmid, pMV-GH, containing rainbow trout growth hormone cDNA fused to mouse metallothionein I promoter, was introduced into medaka (Oryzias latipes) by electroporation. Of 3109 fertilized eggs treated with electric pulses (750 V/cm, 50 μs, 5 times), 783 (25%) hatched out. Four percent of the hatchlings were transgenic. To obtain transgenic lines, 180 hatchlings were maintained and 35 of them grew into adult fish. Two of these fish were transgenic. When one transgenic fish was mated with a normal female, the transgene was found in all the F1 offspring assayed. In F2 offspring obtained by mating transgenic F1 fish, 88% were transgenic. © 1990.

引用

页码：123 / 128

页数：6

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